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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 66, Iss. 11 — Nov. 1, 2012
  • pp: 1294–1301

Fast and Environmentally Friendly Quantitative Analysis of Active Agents in Anti-Diabetic Tablets by an Alternative Laser-Induced Breakdown Spectroscopy (LIBS) Method and Comparison to a Validated Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) Method

Victor Ulises Contreras, Marco A. Meneses-Nava, Nancy Ornelas-Soto, Oracio Barbosa-García, Pedro L. López-de-Alba, José L. Maldonado, Gabriel Ramos-Ortiz, Francisco J. Acevedo-Aguilar, and Leticia López-Martínez

Applied Spectroscopy, Vol. 66, Issue 11, pp. 1294-1301 (2012)


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Abstract

Laser-induced breakdown spectroscopy (LIBS) is evaluated as a potential analytic technique for rapid screening and quality control of anti-diabetic tablets. This paper proposes a simple LIBS-based method for the quantitative analysis of two active pharmaceutical ingredients (APIs): metformin (Met) and glybenclamide (Gly). In order to quantify both APIs, chlorine (Cl) concentration was estimated by employing the Cl/Br optical emission ratio, where Br was introduced as internal standard. Calibration curves were prepared, achieving linearity higher than 99%. On the other hand, for comparison to the proposed method, an isocratic reversed-phase high-performance liquid chromatography (RP-HPLC) method was also developed for quantitative determination of the same analytes by ultraviolet (UV) detection. The chromatographic separation was achieved on a Phenomenex Hypersil C18, 250 mm × 4.6 mm, 5 ?m column. The mobile phase was K<sub>2</sub>HPO<sub>4</sub>/H<sub>3</sub>PO<sub>4</sub>-CH<sub>3</sub>OH and flow rate was 1.0 mL min<sup>?1</sup>. The method is linear over a range of 10-60 ?g mL<sup>?1</sup> for Gly and 5-30 ?g mL<sup>?1</sup> for Met and the correlation coefficients were ?0.99. Recoveries were found to be in the range of 95-101%. Furthermore, four different commercial brands of each active agent were evaluated by both proposed LIBS and chromatographic methods and results were compared with each other. The comparison was satisfactorily validated by analysis of variance (ANOVA).

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Citation
Victor Ulises Contreras, Marco A. Meneses-Nava, Nancy Ornelas-Soto, Oracio Barbosa-García, Pedro L. López-de-Alba, José L. Maldonado, Gabriel Ramos-Ortiz, Francisco J. Acevedo-Aguilar, and Leticia López-Martínez, "Fast and Environmentally Friendly Quantitative Analysis of Active Agents in Anti-Diabetic Tablets by an Alternative Laser-Induced Breakdown Spectroscopy (LIBS) Method and Comparison to a Validated Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) Method," Appl. Spectrosc. 66, 1294-1301 (2012)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-66-11-1294

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