OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 66, Iss. 11 — Nov. 1, 2012
  • pp: 1302–1310

Chemometric Method of Spectra Analysis Leading to Isolation of Lysozyme and CtDNA Spectra Affected by Osmolytes

Piotr Bruździak, Paulina W. Rakowska, and Janusz Stangret

Applied Spectroscopy, Vol. 66, Issue 11, pp. 1302-1310 (2012)


View Full Text Article

Acrobat PDF (904 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

In this paper we present a chemometric method of analysis leading to isolation of Fourier transform infrared (FT-IR) spectra of biomacromolecules (HEW lysozyme, ctDNA) affected by osmolytes (trimethylamine-N-oxide and N,N,N-trimethylglycine, respectively) in aqueous solutions. The method is based on the difference spectra method primarily used to characterize the structure of solvent affected by solute. The cyclical usage of factor analysis allows precise information to be obtained on the shape of “affected spectra” of analyzed biomacromolecules. “Affected spectra” of selected biomacromolecules give valuable information on their structure in the presence of the osmolytes in solution, as well as on the level of perturbation in dependence of osmolyte concentration. The method also gives a possibility of insight into the mechanism of interaction in presented types of systems. It can be easily adapted to various chemical and biochemical problems where vibrational or ultraviolet-visible (UV-Vis) spectroscopy is used.

Virtual Issues
Vol. 7, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Piotr Bruździak, Paulina W. Rakowska, and Janusz Stangret, "Chemometric Method of Spectra Analysis Leading to Isolation of Lysozyme and CtDNA Spectra Affected by Osmolytes," Appl. Spectrosc. 66, 1302-1310 (2012)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-66-11-1302

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited