We report on the optimization of a recently proposed mid-infrared chemical imaging (IRCI) detection method for the analysis of DNA microarrays. The improved protocol allowed for a ten-fold reduction in the time needed to generate a mosaic image of an entire microarray and the production of IR images with high contrast that would facilitate data analysis and interpretation. Advantages of using this protocol were evaluated by applying it to the analysis of four virulence genes in the genomes of 19 strains of the food bacterial pathogen Yersinia enterocolitica.
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics
Brianna Schoen, Magdi M. Mossoba, Vladimir Chizhikov, Anis Rashid, Karen Martinez-Diaz, and Sufian F. Al-Khaldi, "Enhanced Mid-Infrared Chemical Imaging (IRCI) Detection of DNA Microarrays," Appl. Spectrosc. 66, 1480-1486 (2012)
References are not available for this paper.
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.