Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 67,
  • Issue 1,
  • pp. 106-111
  • (2013)

A Sensitive Spectrofluorometric Method for Determination of Ergosta-4,6,8(14),22-Tetraen-3-One in Rat Plasma, Feces, and Urine for Application to Pharmacokinetic Studies Using Cerium(III) as a Probe

Not Accessible

Your library or personal account may give you access

Abstract

Ergosta-4,6,8(14),22-tetraen-3-one (ergone) isolated from <i>Polyporus umbellatus</i> possesses a variety of pharmacological activities in vivo and in vitro, including cytotoxic, diuretic, and immunosuppressive effect. The interaction of cerium ions (Ce<sup>3+</sup>) with ergone was studied by fluorescence and absorption spectroscopy. Spectra data revealed that Ce<sup>3+</sup> ions exhibited emission maxima around 350 nm when the excitation wavelength was fixed at 255 or 290 nm, and the fluorescence of Ce<sup>3+</sup> ions was quenched by the addition of ergone, indicating that a Ce<sup>3+</sup>-ergone complex was formed. According to the modified Benesi-Hildebrand equation, the binding constant of interaction of Ce<sup>3+</sup> ions with ergone was obtained at room temperature. Based on this, a sensitive spectrofluorometric method using Ce<sup>3+</sup> ions as a probe was applied for the identification and quantification of ergone in rat plasma, feces, and urine. The linear ranges of the calibration curves were 1.31 to 4.50 µM for plasma, 1.12-9.87 µM for feces, and 1.28-3.42 µM for urine, and the ergone recoveries were found to be 97.1 ± 0.9%, 98.2 ± 0.7% and 96.5 ± 1.4% for plasma, feces, and urine, respectively. The intraday and inter-day relative standard deviations were less than 9.7%. The proposed spectrofluorometric method is simple and rapid for the quantitative determination of ergone in rat plasma, feces, and urine, and it is affordable for most laboratories because it has few requirements and uses low cost, easy to operate equipment.

PDF Article
More Like This
Quantitative pharmacokinetic and biodistribution studies for fluorescent imaging agents

Yichen Feng, Sanjana Pannem, Sassan Hodge, Cody Rounds, Kenneth M. Tichauer, Keith D. Paulsen, and Kimberley S. Samkoe
Biomed. Opt. Express 15(3) 1861-1877 (2024)

Spectroscopic properties of cerium-doped aluminosilicate glasses

Andreas Herrmann, Hosam A. Othman, Achraf A. Assadi, Mirko Tiegel, Stefan Kuhn, and Christian Rüssel
Opt. Mater. Express 5(4) 720-732 (2015)

Using CaF2:Eu3+ powder as a luminescent probe to detect Cr2O7 2- ions: a new application on the environmental conservation of an old optical material

Jianfu Pan, Kailing Zhong, Zhen Zhang, Wenting Chen, Yanping Lin, Guoqiang Wang, Lingyun Li, and Yan Yu
Opt. Mater. Express 8(9) 2782-2794 (2018)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved