Abstract
Ergosta-4,6,8(14),22-tetraen-3-one (ergone) isolated from <i>Polyporus
umbellatus</i> possesses a variety of pharmacological
activities in vivo and in vitro, including cytotoxic, diuretic, and
immunosuppressive effect. The interaction of cerium ions (Ce<sup>3+</sup>) with
ergone was studied by fluorescence and absorption spectroscopy. Spectra data
revealed that Ce<sup>3+</sup> ions exhibited emission maxima around 350 nm when the
excitation wavelength was fixed at 255 or 290 nm, and the fluorescence of
Ce<sup>3+</sup> ions was quenched by the addition of ergone, indicating that a
Ce<sup>3+</sup>-ergone complex was formed. According to the modified
Benesi-Hildebrand equation, the binding constant of interaction of Ce<sup>3+</sup>
ions with ergone was obtained at room temperature. Based on this, a sensitive
spectrofluorometric method using Ce<sup>3+</sup> ions as a probe was applied for the
identification and quantification of ergone in rat plasma, feces, and urine. The
linear ranges of the calibration curves were 1.31 to 4.50 µM for plasma, 1.12-9.87
µM for feces, and 1.28-3.42 µM for urine, and the ergone recoveries were found to be
97.1 ± 0.9%, 98.2 ± 0.7% and 96.5 ± 1.4% for plasma, feces, and urine, respectively.
The intraday and inter-day relative standard deviations were less than 9.7%. The
proposed spectrofluorometric method is simple and rapid for the quantitative
determination of ergone in rat plasma, feces, and urine, and it is affordable for
most laboratories because it has few requirements and uses low cost, easy to operate
equipment.
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