The liver performs various functions, such as the production and detoxification of chemicals; therefore, it is susceptible to hepatotoxins such as carbon tetrachloride (CCl<sub>4</sub>), which causes chronic injury. Thus, assessment of injury and its status of severity are of prime importance. Current work reports an ex vivo study for probing the severance of hepatic injury induced by CCl<sub>4</sub> with polarized light over the spectral range 400-800 nm. Different concentrations of CCl<sub>4</sub> were used to induce varying severity of hepatic injury in a rat model. Linear retardance, depolarization rates, and diagonal Mueller matrix elements (m<sub>22</sub>, m<sub>33</sub>, and m<sub>44</sub>), were successfully used as the distinguishing criterion for normal and different liver injuries. Our results show that linear retardance for injured liver samples with lower doses of CCl<sub>4</sub> tends to increase when compared with normal liver samples, while samples injured at higher doses of CCl<sub>4</sub> offer almost no retardance. Total, linear, and circular depolarizations follow decreasing trends with increased liver injury severity over the entire investigated wavelength range. Linear polarization states were observed to be better maintained as compared to circular polarization states for all samples. Furthermore, numerical values of diagonal elements of the experimentally measured Mueller matrix also increase with increasing doses of CCl<sub>4</sub>. Liver fibroses, change in transport albedo, and the relative refractive index of the extracellular matrix caused by CCl<sub>4</sub> are responsible for the observed differences. These results will provide a pathway to gauge the severity of injury caused by toxic chemicals.
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics
Manzoor Ahmad, Safdar Ali, Malik Sajjad Mehmood, Hamid Ali, Ahmat Khurshid, Shamaraz Firdous, Saleh Muhammad, and Masroor Ikram, "Ex Vivo Assessment of Carbon Tetrachloride (CCl4)-Induced Chronic Injury Using Polarized Light Spectroscopy," Appl. Spectrosc. 67, 1382-1389 (2013)