Abstract

A simple and selective derivative spectrophotometric method was developed for the quantitative determination of faropenem in pure form and in pharmaceutical dosage. The method is based on the zero-crossing effect of first-derivative spectrophotometry (? = 324 nm), which eliminates the overlapping effect caused by the excipients present in the pharmaceutical preparation, as well as degradation products, formed during hydrolysis, oxidation, photolysis, and thermolysis. The method was linear in the concentration range 2.5-300 ?g/mL (<i>r</i> = 0.9989) at ? = 341 nm; the limits of detection and quantitation were 0.16 and 0.46 ?g/mL, respectively. The method had good precision (relative standard deviation from 0.68 to 2.13%). Recovery of faropenem ranged from 97.9 to 101.3%. The first-order rate constants of the degradation of faropenem in pure form and in pharmaceutical dosage were determined by using first-derivative spectrophotometry. A statistical comparison of the validation results and the observed rate constants for faropenem degradation with these obtained with the high-performance liquid chromatography method demonstrated that both were compatible.

Derivative Absorption and Emission Spectrophotometry

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Appl. Opt. 11(1) 93-98 (1972)

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Comment on: Derivative Absorption and Emission Spectrophotometry

David T. Williams
Appl. Opt. 11(7) 1658-1659 (1972)