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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 2 — Sep. 1, 2010
  • pp: 641–657

Repeatability of tissue fluorescence measurements for the detection of cervical intraepithelial neoplasia

José-Miguel Yamal, Dennis D. Cox, E. Neely Atkinson, Calum MacAulay, Roderick Price, and Michele Follen  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 2, pp. 641-657 (2010)

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We examined intensity and shape differences in 378 repeated spectroscopic measures of the cervix. We examined causes of variability such as presence of precancer or cancer, pathologic tissue type, menopausal status, hormone or oral contraceptive use, and age; as well as technology related variables like generation of device and provider making exam. Age, device generation, and provider were statistically significantly related to intensity differences. Provider and device generation were related to shape differences. We examined the order of measurements and found a decreased intensity in the second measurement due to hemoglobin absorption. 96% of repeat measurements had classification concordance of cervical intraepithelial neoplasia.

© 2010 OSA

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(300.0300) Spectroscopy : Spectroscopy

ToC Category:
Optics in Cancer Research

Original Manuscript: June 2, 2010
Revised Manuscript: August 5, 2010
Manuscript Accepted: August 17, 2010
Published: August 19, 2010

Virtual Issues
Bio-Optics in Clinical Application, Nanotechnology, and Drug Discovery (2010) Biomedical Optics Express

José-Miguel Yamal, Dennis D. Cox, E. Neely Atkinson, Calum MacAulay, Roderick Price, and Michele Follen, "Repeatability of tissue fluorescence measurements for the detection of cervical intraepithelial neoplasia," Biomed. Opt. Express 1, 641-657 (2010)

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  1. J. Freeberg, D. Serachitopol, N. McKinnon, R. Price, E. N. Atkinson, D. D. Cox, C. MacAulay, R. Richards-Kortum, M. Follen, and B. Pikkula, “Fluorescence and reflectance device variability throughout the progression of a phase II clinical trial to detect and screen for cervical neoplasia using a fiber optic probe,” J. Biomed. Opt. 12(3), 034015 (2007). [CrossRef]
  2. N. Marín, N. MacKinnon, C. MacAulay, S. K. Chang, E. N. Atkinson, D. D. Cox, D. Serachitopol, B. Pikkula, M. Follen, and R. Richards-Kortum, “Calibration standards for multicenter clinical trials of fluorescence spectroscopy for in vivo diagnosis,” J. Biomed. Opt. 11(1), 014010 (2006). [CrossRef]
  3. S.B. Cantor, J.M. Yamal, M. Guillaud, D.D. Cox, E.N. Atkinson, J.L. Benedet, D. Miller, T. Ehlen, J. Matisic, D. van Niekerk, M. Bertrand, A. Milbourne, H. Rhodes, A. Malpica, G. Staerkel, S. Nader-Eftekhari, K. Adler-Storthz, M.E. Scheurer, K. Basen-Engquist, E. Shinn, L.A. West, A.T. Vlastos, X. Tao, J.R. Beck, C. MacAulay, and M. Follen, “Accuracy of optical spectroscopy for the detection of cervical intraepithelial neoplasia: testing a device as an adjunct to colposcopy,” Int. J. Cancer (accepted for resubmission).
  4. S. K. Chang, M. Dawood, G. Staerkel, U. Utzinger, E. N. Atkinson, R. Richards-Kortum, and M. Follen, “Fluorescence spectroscopy for cervical precancer detection: Is there variance across the menstrual cycle?” J. Biomed. Opt. 7(4), 595–602 (2002). [CrossRef]
  5. D. D. Cox, S. K. Chang, M. Dawood, G. Staerkel, U. Utzinger, R. Richards-Kortum, and M. Follen, “Detecting the signal of the menstrual cycle in fluorescence spectroscopy of the cervix,” Appl. Spectrosc. 57(1), 67–72 (2003). [CrossRef]
  6. A. Nath, K. Rivoire, S. Chang, D. Cox, E. N. Atkinson, M. Follen, and R. Richards-Kortum, “Effect of probe pressure on cervical fluorescence spectroscopy measurements,” J. Biomed. Opt. 9(3), 523–533 (2004). [CrossRef]
  7. K. Rivoire, A. Nath, D. D. Cox, E. N. Atkinson, R. Richards-Kortum, and M. Follen, “The effects of repeated spectroscopic pressure measurements on fluorescence intensity in the cervix,” Am. J. Obstet. Gynecol. 191(5), 1606–1617 (2004). [CrossRef]
  8. C. Brookner, U. Utzinger, M. Follen, R. Richards-Kortum, D. D. Cox, and E. N. Atkinson, “Effects of biographical variables on cervical fluorescence emission spectra,” J. Biomed. Opt. 8(3), 479–483 (2003). [CrossRef]
  9. C. Brookner, M. Follen, I. Boiko, J. Galvan, S. Thomsen, A. Malpica, S. Suzuki, R. Lotan, and R. Richards-Kortum, “Autofluorescence patterns in short-term cultures of normal cervical tissue,” Photochem. Photobiol. 71(6), 730–736 (2000). [CrossRef]
  10. N. Ramanujam, R. Richards-Kortum, S. Thomsen, A. Mahadevan-Jansen, M. Follen, and B. Chance, “Low Temperature Fluorescence Imaging of Freeze-trapped Human Cervical Tissues,” Opt. Express 8(6), 335–343 (2001). [CrossRef]
  11. R. Drezek, C. Brookner, I. Pavlova, I. Boiko, A. Malpica, R. Lotan, M. Follen, and R. Richards-Kortum, “Autofluorescence microscopy of fresh cervical-tissue sections reveals alterations in tissue biochmistry with dysplasia,” Photochem. Photobiol. 73(6), 636–641 (2001). [CrossRef]
  12. R. Drezek, K. Sokolov, U. Utzinger, I. Boiko, A. Malpica, M. Follen, and R. Richards-Kortum, “Understanding the contributions of NADH and collagen to cervical tissue fluorescence spectra: modeling, measurements, and implication,” J. Biomed. Opt. 6(4), 385–396 (2001). [CrossRef]
  13. R. Drezek, M. Guillaud, T. Collier, I. Boiko, A. Malpica, C. Macaulay, M. Follen, and R. Richards-Kortum, “Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture,” J. Biomed. Opt. 8(1), 7–16 (2003). [CrossRef]
  14. D. Arifler, I. Pavlova, A. Gillenwater, and R. Richards-Kortum, “Light scattering from collagen fiber networks: micro-optical properties of normal and neoplastic stroma,” Biophys. J. 92(9), 3260–3274 (2007). [CrossRef]
  15. I. Pavlova, K. Sokolov, R. Drezek, A. Malpica, M. Follen, and R. Richards-Kortum, “Microanatomical and biochemical origins of normal and precancerous cervical autofluorescence using laser-scanning fluorescence confocal microscopy,” Photochem. Photobiol. 77(5), 550–555 (2003). [CrossRef]
  16. J. S. Lee, O. Shuhatovich, R. Price, B. Pikkula, M. Follen, N. McKinnon, C. Macaulay, B. Knight, R. Richards-Kortum, and D. D. Cox, “Design and preliminary analysis of a study to assess intra-device and inter-device variability of fluorescence spectroscopy instruments for detecting cervical neoplasia,” Gynecol. Oncol. 99(3), S98–S111 (2005). [CrossRef]
  17. B. M. Pikkula, O. Shuhatovich, R. L. Price, D. M. Serachitopol, M. Follen, N. McKinnon, C. MacAulay, R. Richards-Kortum, J. S. Lee, E. N. Atkinson, and D. D. Cox, “Instrumentation as a source of variability in the application of fluorescence spectroscopic devices for detecting cervical neoplasia,” J. Biomed. Opt. 12(3), 034014 (2007). [CrossRef]
  18. B. Pikkula, D. Serachitopol, C. MacAulay, N. Mackinnon, J. S. Lee, D. D. Cox, E. N. Atkinson, M. Follen, and R. Richards-Kortum, “Multicenter clinical trials of in vivo fluorescence: are the measurements equivalent?” Proc SPIE 6430–64301Q (2007).
  19. D. M. Gershensen, A. H. DeCherney, S. L. Curry, and L. Brubaker, Operative Gynecology, 2nd edition, (Saunders, 2001).
  20. H. Zhu, and D. D. Cox, “A Functional Generalized Linear Model with Curve Selection in Cervical Pre-cancer Diagnosis Using Fluorescence Spectroscopy,” Optimality: The Third Erich L. Lehmann Symposium 57, 173–189 (2009).
  21. H. Zhu, M. Vannucci, and D. D. Cox, “A Bayesian Hierarchical Model for Classification with Selection of Functional Predictors,” Biometrics 66(2), 463–473 (2010). [CrossRef]

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