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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 2 — Sep. 1, 2010
  • pp: 676–686

Ultrahigh resolution photoacoustic microscopy via transient absorption

Ryan L. Shelton and Brian E. Applegate  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 2, pp. 676-686 (2010)
http://dx.doi.org/10.1364/BOE.1.000676


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Abstract

We have developed a novel, hybrid imaging modality, Transient Absorption Ultrasonic Microscopy (TAUM), which takes advantage of the optical nonlinearities afforded by transient absorption to achieve ultrahigh-resolution photoacoustic microscopy. The theoretical point spread function for TAUM is functionally equivalent to confocal and two-photon fluorescence microscopy, potentially enabling cellular/subcellular photoacoustic imaging. A prototype TAUM system was designed, built, and used to image a cross-section through several capillaries in the excised cheek pouch of a Syrian Hamster. The well-resolved capillaries in the TAUM image provided experimental evidence of the spatial resolution. These results suggest that TAUM has excellent potential for producing volumetric images with cellular/subcellular resolution in three dimensions deep inside living tissue.

© 2010 OSA

OCIS Codes
(110.5120) Imaging systems : Photoacoustic imaging
(180.4315) Microscopy : Nonlinear microscopy
(110.5125) Imaging systems : Photoacoustics

ToC Category:
Photoacoustic Imaging and Spectroscopy

History
Original Manuscript: June 1, 2010
Revised Manuscript: August 12, 2010
Manuscript Accepted: August 14, 2010
Published: August 23, 2010

Virtual Issues
Advances in Optical Coherence Tomography, Photoacoustic Imaging, and Microscopy (2010) Biomedical Optics Express

Citation
Ryan L. Shelton and Brian E. Applegate, "Ultrahigh resolution photoacoustic microscopy via transient absorption," Biomed. Opt. Express 1, 676-686 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-2-676


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