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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 2 — Sep. 1, 2010
  • pp: 687–693

Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy

Keisuke Isobe, Hiroshi Hashimoto, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 2, pp. 687-693 (2010)
http://dx.doi.org/10.1364/BOE.1.000687


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Abstract

We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form.

© 2010 OSA

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.6420) Spectroscopy : Spectroscopy, nonlinear

ToC Category:
Molecular Imaging and Probe Development

History
Original Manuscript: June 11, 2010
Revised Manuscript: August 11, 2010
Manuscript Accepted: August 18, 2010
Published: August 23, 2010

Citation
Keisuke Isobe, Hiroshi Hashimoto, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa, "Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy," Biomed. Opt. Express 1, 687-693 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-2-687


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