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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 5 — Dec. 1, 2010
  • pp: 1320–1330

Feasibility of using multiphoton excited tissue autofluorescence for in vivo human histopathology.

Johanna M. Dela Cruz, Jesse D. McMullen, Rebecca M. Williams, and Warren R. Zipfel  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 5, pp. 1320-1330 (2010)

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Rapid and direct imaging of microscopic tissue morphology and pathology can be achieved by multiphoton imaging of intrinsic tissue fluorophores and second harmonic signals. Engineering parameters for developing this technology for clinical applications include excitation levels and collection efficiencies required to obtain diagnostic quality images from different tissue types and whether these levels are mutagenic. Here we provide data on typical average powers required for high signal-to-noise in vivo tissue imaging and assess the risk potential of these irradiance levels using a mammalian cell gene mutation assay. Exposure times of ~16 milliseconds per cell to 760 nm, ~200 fs raster-scanned laser irradiation delivered through a 0.75 NA objective produced negligible mutagenicity at powers up to about 50 mW.

© 2010 OSA

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: September 15, 2010
Revised Manuscript: October 27, 2010
Manuscript Accepted: October 29, 2010
Published: November 5, 2010

Johanna M. Dela Cruz, Jesse D. McMullen, Rebecca M. Williams, and Warren R. Zipfel , "Feasibility of using multiphoton excited tissue autofluorescence for in vivo human histopathology.," Biomed. Opt. Express 1, 1320-1330 (2010)

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