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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 1 — Jan. 1, 2011
  • pp: 71–79

Two-photon excited hemoglobin fluorescence

Wei Zheng, Dong Li, Yan Zeng, Yi Luo, and Jianan Y. Qu  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 1, pp. 71-79 (2011)

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We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels.

© 2010 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.6410) Spectroscopy : Spectroscopy, multiphoton
(300.6500) Spectroscopy : Spectroscopy, time-resolved
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Spectroscopic Diagnostics

Original Manuscript: October 27, 2010
Revised Manuscript: December 1, 2010
Manuscript Accepted: December 2, 2010
Published: December 6, 2010

Wei Zheng, Dong Li, Yan Zeng, Yi Luo, and Jianan Y. Qu, "Two-photon excited hemoglobin fluorescence," Biomed. Opt. Express 2, 71-79 (2011)

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