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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 10 — Oct. 1, 2011
  • pp: 2784–2791

Born approximation model for light scattering by red blood cells

Joonoh Lim, Huafeng Ding, Mustafa Mir, Ruoyu Zhu, Krishnarao Tangella, and Gabriel Popescu  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 10, pp. 2784-2791 (2011)
http://dx.doi.org/10.1364/BOE.2.002784


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Abstract

The primary role of a red blood cell (RBC) is delivering oxygen throughout our body. Abnormalities of this basic function lead to anemia and are caused by numerous diseases such as malaria and sickle cell anemia. As prompt and inexpensive tests for blood screening are in demand, we have developed a faster and reliable way to measure morphological parameters associated with the structure of red blood cells and the size distribution of the cells in a whole blood smear. Modeling the RBC shape under Born approximation, we are able to determine parameters of clinical relevance, such as the diameter, thickness and dimple size. From a measured quantitative phase image of a blood smear, we can determine the average and standard deviation of the red blood cell volume simultaneously, i.e., without analyzing each cell individually. This approach may open the door for a new generation of label-free, high-throughput blood testing.

© 2011 OSA

OCIS Codes
(100.2960) Image processing : Image analysis
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(180.3170) Microscopy : Interference microscopy
(070.7345) Fourier optics and signal processing : Wave propagation

ToC Category:
Cell Studies

History
Original Manuscript: July 29, 2011
Revised Manuscript: September 6, 2011
Manuscript Accepted: September 9, 2011
Published: September 13, 2011

Virtual Issues
Advances in Optics for Biotechnology, Medicine, and Surgery (2011) Biomedical Optics Express

Citation
Joonoh Lim, Huafeng Ding, Mustafa Mir, Ruoyu Zhu, Krishnarao Tangella, and Gabriel Popescu, "Born approximation model for light scattering by red blood cells," Biomed. Opt. Express 2, 2784-2791 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-10-2784


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