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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 10 — Oct. 1, 2011
  • pp: 2815–2820

Simultaneous optical measurements of cell motility and growth

Shamira Sridharan, Mustafa Mir, and Gabriel Popescu  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 10, pp. 2815-2820 (2011)

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It has recently been shown that spatial light interference microscopy (SLIM) developed in our laboratory can be used to quantify the dry mass growth of single cells with femtogram sensitivity [M. Mir et al., Proc. Nat. Acad. Sci. 108, 32 (2011)]. Here we show that it is possible to measure the motility of single cells in conjunction with the dry mass measurements. Specifically the effect of poly-L-lysine substrate on the dry mass growth of Drosophila S2 cells is studied. By measuring the mean square displacement of single cells and clusters it is shown that cells that adhere better to the surface are unable to grow. Using such a technique it is possible to measure both growth and morphogenesis, two of the cornerstones of developmental biology.

© 2011 OSA

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy

ToC Category:
Cell Studies

Original Manuscript: July 27, 2011
Revised Manuscript: September 16, 2011
Manuscript Accepted: September 18, 2011
Published: September 22, 2011

Virtual Issues
Advances in Optics for Biotechnology, Medicine, and Surgery (2011) Biomedical Optics Express

Shamira Sridharan, Mustafa Mir, and Gabriel Popescu, "Simultaneous optical measurements of cell motility and growth," Biomed. Opt. Express 2, 2815-2820 (2011)

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