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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 2 — Feb. 1, 2011
  • pp: 264–271

Superresolved femtosecond laser nanosurgery of cells

Matthias Pospiech, Moritz Emons, Kai Kuetemeyer, Alexander Heisterkamp, and Uwe Morgner  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 2, pp. 264-271 (2011)
http://dx.doi.org/10.1364/BOE.2.000264


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Abstract

We report on femtosecond nanosurgery of fluorescently labeled structures in cells with a spatially superresolved laser beam. The focal spot width is reduced using phase filtering applied with a programmable phase modulator. A comprehensive statistical analysis of the resulting cuts demonstrates an achievable average resolution enhancement of 30 %.

© 2011 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.0180) Medical optics and biotechnology : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Microscopy

History
Original Manuscript: November 4, 2010
Revised Manuscript: December 21, 2010
Manuscript Accepted: December 22, 2010
Published: January 5, 2011

Citation
Matthias Pospiech, Moritz Emons, Kai Kuetemeyer, Alexander Heisterkamp, and Uwe Morgner, "Superresolved femtosecond laser nanosurgery of cells," Biomed. Opt. Express 2, 264-271 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-2-264


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