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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 3 — Mar. 1, 2011
  • pp: 579–591

Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography

Zhongwei Zhi, William Cepurna, Elaine Johnson, Tueng Shen, John Morrison, and Ruikang K Wang  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 3, pp. 579-591 (2011)
http://dx.doi.org/10.1364/BOE.2.000579


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Abstract

In this paper, we present methods for 3D visualization and quantitative measurements of retinal blood flow in rats by the use of optical microangiography imaging technique (OMAG). We use ultrahigh sensitive OMAG to provide high-quality 3D RBF perfusion maps in the rat eye, from which the Doppler angle, as well as the diameters of blood vessels, are evaluated. Estimation of flow velocity (i.e. axial flow velocity) is achieved by the use of Doppler OMAG, which has its origins in phase-resolved Doppler optical coherence tomography. The measurements of the Doppler angle, vessel size, and the axial velocity lead to the quantitative assessment of the absolute flow velocity and the blood flow rate in selected retinal vessels. We demonstrate the feasibility of OMAG to provide 3D microangiograms and quantitative assessment of retinal blood flow in a rat model subjected to raised intra-ocular pressure (IOP). We show that OMAG is capable of monitoring the longitudinal response of absolute blood velocity and flow rate of retinal blood vessels to increased IOP in the rat, demonstrating its usefulness for ophthalmological research.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: January 4, 2011
Manuscript Accepted: February 10, 2011
Published: February 15, 2011

Virtual Issues
In vivo Microcirculation Imaging (2011) Biomedical Optics Express

Citation
Zhongwei Zhi, William Cepurna, Elaine Johnson, Tueng Shen, John Morrison, and Ruikang K Wang, "Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography," Biomed. Opt. Express 2, 579-591 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-3-579


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