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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 4 — Apr. 1, 2011
  • pp: 937–945

Chemical visualization of individual chondrocytes in articular cartilage by attenuated-total-reflection Fourier Transform Infrared Microimaging

Jianhua Yin and Yang Xia  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 4, pp. 937-945 (2011)

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Fourier transform infrared imaging (FTIRI) and the attenuated total reflection Fourier transform infrared microimaging (ATR-FTIRM) were used to study the chemical and structural distributions of cellular components surrounding individual chondrocytes in canine humeral cartilage, at 6.25µm pixel resolution in FTIRI and 1.56µm pixel resolution in ATR-FTIRM. The chemical and structural distributions of the cellular components in chondrocytes and tissue can be successfully imaged in high resolution ATR-FTIRM. One can also study the territorial matrix of fine collagen fibrils surrounding the individual chondrocytes by the polarization experiments using the absorption ratio of amide I to amide II bands.

© 2011 OSA

OCIS Codes
(110.3080) Imaging systems : Infrared imaging
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(300.6340) Spectroscopy : Spectroscopy, infrared

ToC Category:
Cell Studies

Original Manuscript: December 20, 2010
Revised Manuscript: February 25, 2011
Manuscript Accepted: March 7, 2011
Published: March 18, 2011

Jianhua Yin and Yang Xia, "Chemical visualization of individual chondrocytes in articular cartilage by attenuated-total-reflection Fourier Transform Infrared Microimaging," Biomed. Opt. Express 2, 937-945 (2011)

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