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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 5 — May. 1, 2011
  • pp: 1082–1096

Spectral-profile-based algorithm for hemoglobin oxygen saturation determination from diffuse reflectance spectra

Po-Ching Chen and Wei-Chiang Lin  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 5, pp. 1082-1096 (2011)

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Variations of hemoglobin (Hb) oxygenation in tissue provide important indications concerning the physiological conditions of tissue, and the data related to these variations are of intense interest in medical research as well as in clinical care. In this study, we derived a new algorithm to estimate Hb oxygenation from diffuse reflectance spectra. The algorithm was developed based on the unique spectral profile differences between the extinction coefficient spectra of oxy-Hb and deoxy-Hb within the visible wavelength region. Using differential wavelet transformation, these differences were quantified using the locations of certain spectral features, and, then, they were related to the oxygenation saturation level of Hb. The applicability of the algorithm was evaluated using a set of diffuse reflectance spectra produced by a Monte Carlo simulation model of photon migration and by tissue phantoms experimentally. The algorithm was further applied to the diffuse reflectance spectra acquired from in vivo experiments to demonstrate its clinical utility. The validation and evaluation results concluded that the algorithm is applicable to various tissue types (i.e., scattering properties) and can be easily used in conjunction with a diverse range of probe geometries for real-time monitoring of Hb oxygenation.

© 2011 OSA

OCIS Codes
(070.4790) Fourier optics and signal processing : Spectrum analysis
(170.1610) Medical optics and biotechnology : Clinical applications
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Spectroscopic Diagnostics

Original Manuscript: January 4, 2011
Revised Manuscript: March 29, 2011
Manuscript Accepted: April 1, 2011
Published: April 5, 2011

Po-Ching Chen and Wei-Chiang Lin, "Spectral-profile-based algorithm for hemoglobin oxygen saturation determination from diffuse reflectance spectra," Biomed. Opt. Express 2, 1082-1096 (2011)

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