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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 5 — May. 1, 2011
  • pp: 1233–1242

Dual-channel imaging system for singlet oxygen and photosensitizer for PDT

Seonkyung Lee, Martin E. Isabelle, Kristin L. Gabally-Kinney, Brian W. Pogue, and Steven J. Davis  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 5, pp. 1233-1242 (2011)
http://dx.doi.org/10.1364/BOE.2.001233


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Abstract

A two-channel optical system has been developed to provide spatially resolved simultaneous imaging of singlet molecular oxygen (1O2) phosphorescence and photosensitizer (PS) fluorescence produced by the photodynamic process. The current imaging system uses a spectral discrimination method to differentiate the weak 1O2 phosphorescence that peaks near 1.27 μm from PS fluorescence that also occurs in this spectral region. The detection limit of 1O2 emission was determined at a concentration of 500 nM benzoporphyrin derivative monoacid (BPD) in tissue-like phantoms, and these signals observed were proportional to the PS fluorescence. Preliminary in vivo images with tumor laden mice indicate that it is possible to obtain simultaneous images of 1O2 and PS tissue distribution.

© 2011 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5180) Medical optics and biotechnology : Photodynamic therapy

ToC Category:
Optical Therapies and Photomodificaton

History
Original Manuscript: February 7, 2011
Revised Manuscript: March 30, 2011
Manuscript Accepted: April 13, 2011
Published: April 15, 2011

Citation
Seonkyung Lee, Martin E. Isabelle, Kristin L. Gabally-Kinney, Brian W. Pogue, and Steven J. Davis, "Dual-channel imaging system for singlet oxygen and photosensitizer for PDT," Biomed. Opt. Express 2, 1233-1242 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-5-1233


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