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In vivo fluorescence lifetime optical projection tomographyJames McGinty, Harriet B. Taylor, Lingling Chen, Laurence Bugeon, Jonathan R. Lamb, Margaret J. Dallman, and Paul M. W. French »View Author Affiliations
James McGinty,1,*
Harriet B. Taylor,2
Lingling Chen,1
Laurence Bugeon,2
Jonathan R. Lamb,2
Margaret J. Dallman,2,3
and Paul M. W. French1
1Photonics Group, Department of Physics, Imperial College London, SW7 2AZ, UK 2Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, SW7 2AZ, UK 3Centre for Integrative Systems Biology, Department of Life Sciences, Imperial College London, SW7 2AZ, UK *Corresponding author: james.mcginty@imaperial.co.uk |
Biomedical Optics Express, Vol. 2, Issue 5, pp. 1340-1350 (2011)
http://dx.doi.org/10.1364/BOE.2.001340
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Abstract
We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.
© 2011 OSA
OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.6960) Medical optics and biotechnology : Tomography
ToC Category:
Microscopy
History
Original Manuscript: March 4, 2011
Revised Manuscript: April 21, 2011
Manuscript Accepted: April 21, 2011
Published: April 26, 2011
Virtual Issues
July 22, 2011 Spotlight on Optics
Citation
James McGinty, Harriet B. Taylor, Lingling Chen, Laurence Bugeon, Jonathan R. Lamb, Margaret J. Dallman, and Paul M. W. French, "In vivo fluorescence lifetime optical projection tomography," Biomed. Opt. Express 2, 1340-1350 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-5-1340
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References
- R. Y. Tsien, “The green fluorescent protein,” Annu. Rev. Biochem. 67(1), 509–544 (1998). [CrossRef] [PubMed]
- P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999). [CrossRef] [PubMed]
- E. A. Jares-Erijman and T. M. Jovin, “FRET imaging,” Nat. Biotechnol. 21(11), 1387–1395 (2003). [CrossRef] [PubMed]
- M. Mank, D. F. Reiff, N. Heim, M. W. Friedrich, A. Borst, and O. Griesbeck, “A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change,” Biophys. J. 90(5), 1790–1796 (2006). [CrossRef] [PubMed]
- H. Ueyama, M. Takagi, and S. Takenaka, “A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation,” J. Am. Chem. Soc. 124(48), 14286–14287 (2002). [CrossRef] [PubMed]
- T. Kuner and G. J. Augustine, “A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons,” Neuron 27(3), 447–459 (2000). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
- A. Nezu, A. Tanimura, T. Morita, A. Shitara, and Y. Tojyo, “A novel fluorescent method employing the FRET-based biosensor “LIBRA” for the identification of ligands of the inositol 1,4,5-trisphosphate receptors,” Biochim. Biophys. Acta 1760(8), 1274–1280 (2006). [PubMed]
- T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda, “Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells,” Mol. Biol. Cell 19(10), 4213–4223 (2008). [CrossRef] [PubMed]
- I. T. Li, E. Pham, and K. Truong, “Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics,” Biotechnol. Lett. 28(24), 1971–1982 (2006). [CrossRef] [PubMed]
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
- X. F. Wang, A. Periasamy, B. Herman, and D. M. Coleman, “Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications,” Crit. Rev. Anal. Chem. 23(5), 369–395 (1992). [CrossRef]
- K. Dowling, S. C. W. Hyde, J. C. Dainty, P. W. W. French, and J. D. Hares, “2-D fluorescence lifetime imaging using a time-gated image intensifier,” Opt. Commun. 135(1-3), 27–31 (1997). [CrossRef]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- A. Abbott, “Cell culture: biology’s new dimension,” Nature 424(6951), 870–872 (2003). [CrossRef] [PubMed]
- P. J. Keller, F. Pampaloni, and E. H. K. Stelzer, “Life sciences require the third dimension,” Curr. Opin. Cell Biol. 18(1), 117–124 (2006). [CrossRef] [PubMed]
- R. Weissleder and V. Ntziachristos, “Shedding light onto live molecular targets,” Nat. Med. 9(1), 123–128 (2003). [CrossRef] [PubMed]
- D. Stockholm, M. Bartoli, G. Sillon, N. Bourg, J. Davoust, and I. Richard, “Imaging calpain protease activity by multiphoton FRET in living mice,” J. Mol. Biol. 346(1), 215–222 (2005). [CrossRef] [PubMed]
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- C. Dunsby, “Optically sectioned imaging by oblique plane microscopy,” Opt. Express 16(25), 20306–20316 (2008). [CrossRef] [PubMed]
- H. S. Sakhalkar, M. Dewhirst, T. Oliver, Y. Cao, and M. Oldham, “Functional imaging in bulk tissue specimens using optical emission tomography: fluorescence preservation during optical clearing,” Phys. Med. Biol. 52(8), 2035–2054 (2007). [CrossRef] [PubMed]
- M. Oldham, H. S. Sakhalkar, T. Oliver, G. Allan Johnson, and M. Dewhirst, “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography,” J. Biomed. Opt. 13(2), 021113 (2008). [CrossRef] [PubMed]
- J. Huisken and D. Y. R. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32(17), 2608–2610 (2007). [CrossRef] [PubMed]
- C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2008). [CrossRef] [PubMed]
- U. J. Birk, M. Rieckher, N. Konstantinides, A. Darrell, A. Sarasa-Renedo, H. Meyer, N. Tavernarakis, and J. Ripoll, “Correction for specimen movement and rotation errors for in-vivo Optical Projection Tomography,” Biomed. Opt. Express 1(1), 87–96 (2010). [CrossRef] [PubMed]
- D. Beis and D. Y. R. Stainier, “In vivo cell biology: following the zebrafish trend,” Trends Cell Biol. 16(2), 105–112 (2006). [CrossRef] [PubMed]
- C. Parng, W. L. Seng, C. Semino, and P. McGrath, “Zebrafish: a preclinical model for drug screening,” Assay Drug Dev. Technol. 1(1), 41–48 (2002). [CrossRef] [PubMed]
- A. J. Hill, H. Teraoka, W. Heideman, and R. E. Peterson, “Zebrafish as a model vertebrate for investigating chemical toxicity,” Toxicol. Sci. 86(1), 6–19 (2005). [CrossRef] [PubMed]
- T. P. Barros, W. K. Alderton, H. M. Reynolds, A. G. Roach, and S. Berghmans, “Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery,” Br. J. Pharmacol. 154(7), 1400–1413 (2008). [CrossRef] [PubMed]
- C. G. Burns, D. J. Milan, E. J. Grande, W. Rottbauer, C. A. MacRae, and M. C. Fishman, “High-throughput assay for small molecules that modulate zebrafish embryonic heart rate,” Nat. Chem. Biol. 1(5), 263–264 (2005). [CrossRef] [PubMed]
- G. Kari, U. Rodeck, and A. P. Dicker, “Zebrafish: an emerging model system for human disease and drug discovery,” Clin. Pharmacol. Ther. 82(1), 70–80 (2007). [CrossRef] [PubMed]
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- C. Hall, M. V. Flores, T. Storm, K. Crosier, and P. Crosier, “The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish,” BMC Dev. Biol. 7(1), 42 (2007). [CrossRef] [PubMed]
- J. R. Mathias, M. E. Dodd, K. B. Walters, S. K. Yoo, E. A. Ranheim, and A. Huttenlocher, “Characterization of zebrafish larval inflammatory macrophages,” Dev. Comp. Immunol. 33(11), 1212–1217 (2009). [CrossRef] [PubMed]
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- A. C. Kak and M. Slaney, “Principles of computerized tomographic imaging,” R. E. O’Malley ed. (SIAM, IEEE Press, New York, 1988).
- J. I. Agulleiro and J. J. Fernandez, “Fast tomographic reconstruction on multicore computers,” Bioinformatics 27(4), 582–583 (2011). [CrossRef] [PubMed]
- D. Castaño Díez, H. Mueller, and A. S. Frangakis, “Implementation and performance evaluation of reconstruction algorithms on graphics processors,” J. Struct. Biol. 157(1), 288–295 (2007). [CrossRef] [PubMed]
- R. M. Ballew and J. N. Demas, “An error analysis of the rapid lifetime determination method for the evaluation of single exponential decay,” Anal. Chem. 61(1), 30–33 (1989). [CrossRef]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- K. Dowling, M. J. Dayel, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw, “Fluorescence lifetime imaging with picosecond resolution for biomedical applications,” Opt. Lett. 23(10), 810–812 (1998). [CrossRef] [PubMed]
- A. Esposito, H. C. Gerritsen, T. Oggier, F. Lustenberger, and F. S. Wouters, “Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics,” J. Biomed. Opt. 11(3), 034016 (2006). [CrossRef] [PubMed]
- L. K. van Geest and K. W. J. Stoop, “FLIM on a wide field fluorescence microscope,” Lett. Pept. Sci. 10(5-6), 501–510 (2003). [CrossRef]
- J. R. Walls, J. G. Sled, J. Sharpe, and R. M. Henkelman, “Resolution improvement in emission optical projection tomography,” Phys. Med. Biol. 52(10), 2775–2790 (2007). [CrossRef] [PubMed]
- V. Y. Soloviev and S. R. Arridge, “Optical Tomography in weakly scattering media in the presence of highly scattering inclusions,” Biomed. Opt. Express 2(3), 440–451 (2011). [CrossRef] [PubMed]
- C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
Abbott, A.
Agulleiro, J. I.
Ahlgren, U.
Alderton, W. K.
Alexandrov, Y.
Alibhai, D.
Allan Johnson, G.
Aoki, K.
Arridge, S. R.
Augustine, G. J.
Balciunas, D.
Baldock, R.
Ballew, R. M.
Barros, T. P.
Bartoli, M.
Bastiaens, P. I. H.
Becker, K.
Bedell, V. M.
Beis, D.
Berghmans, S.
Birk, U. J.
Boczek, N. J.
Boot, M. J.
Borst, A.
Bourg, N.
Bunney, T. D.
Burns, C. G.
Buurman, E. P.
Cao, Y.
Castaño Díez, D.
Chang, T. Y.
Clark, K. J.
Coleman, D. M.
Cotterell, J.
Crosier, K.
Crosier, P.
Dainty, J. C.
Dallman, M. J.
Darrell, A.
Davidson, D.
Davis, D. M.
Davoust, J.
Dayel, M. J.
Deininger, K.
Del Bene, F.
Demas, J. N.
Deussing, J. M.
Dewhirst, M.
Dicker, A. P.
Dodd, M. E.
Dodt, H. U.
Dowling, K.
Draaijer, A.
Dunsby, C.
Dymoke-Bradshaw, A. K. L.
Eagle, R.
Eder, M.
Eickhoff, J.
Ekker, S. C.
Eliceiri, K. W.
Elson, D. S.
Esposito, A.
Essner, J. J.
Fernandez, J. J.
Fishman, M. C.
Flores, M. V.
Frangakis, A. S.
French, P. M. W.
French, P. W. W.
Friedrich, M. W.
Galletly, N.
Gendron-Fitzpatrick, A.
Gerritsen, H. C.
Gilleland, C. L.
Grande, E. J.
Grant, D.
Grant, D. M.
Griesbeck, O.
Hall, C.
Hares, J. D.
Hargreaves, A.
Hecksher-Sørensen, J.
Heideman, W.
Heim, N.
Henkelman, R. M.
Herman, B.
Hikake, K.
Hill, A. J.
Hill, B.
Houpt, P. M.
Huisken, J.
Huttenlocher, A.
Hyde, S. C. W.
Isherwood, B.
Jährling, N.
Jares-Erijman, E. A.
Jovin, T. M.
Kari, G.
Katan, M.
Keller, P. J.
Kellett, P. A.
Kelly, D.
Kennedy, G.
Kennedy, G. T.
Kimberly, C.
Kiyokawa, E.
Konstantinides, N.
Koo, B. K.
Kumar, S.
Kuner, T.
Kurokawa, K.
Lacombe, F.
Laine, R.
Lam, E. W.-F.
Lanigan, P. M. P.
Leischner, U.
Lever, M. J.
Levine, Y. K.
Li, I. T.
Lustenberger, F.
MacRae, C. A.
Magee, A. I.
Mank, M.
Manning, H. B.
Margineanu, A.
Mathias, J. R.
Matsuda, M.
Mauch, C. P.
McCann, F.
McGhee, E. J.
McGinty, J.
McGrath, P.
Meyer, H.
Milan, D. J.
Miyawaki, A.
Mochizuki, N.
Morita, T.
Mueller, H.
Munro, I.
Murray, E. J.
Nagai, T.
Neil, M A A.
Neil, M. A.
Neil, M. A. A.
Nezu, A.
Nishioka, T.
Ntziachristos, V.
Oggier, T.
Ohba, Y.
Oldham, M.
Oliver, T.
Onfelt, B.
Owen, D. M.
Pampaloni, F.
Pardo-Martin, C.
Parng, C.
Periasamy, A.
Perrimon, N.
Perry, P.
Peterson, R. E.
Petzold, A. M.
Pham, E.
Pitsouli, C.
Quintana, L.
Ramanujam, N.
Ranheim, E. A.
Razansky, D.
Reiff, D. F.
Requejo-Isidro, J.
Reynolds, H. M.
Richard, I.
Riching, K. M.
Rieckher, M.
Ripoll, J.
Roach, A. G.
Rodeck, U.
Ross, A.
Rottbauer, W.
Sakhalkar, H. S.
Sanders, R.
Sanz-Ezquerro, J.
Sarasa-Renedo, A.
Sardini, A.
Schierloh, A.
Schweitzer, R.
Semino, C.
Seng, W. L.
Sharpe, J.
Shitara, A.
Sillon, G.
Skala, M. C.
Sled, J. G.
Soloviev, V. Y.
Squire, A.
Stainier, D. Y. R.
Stamp, G.
Stamp, G. W.
Stelzer, E. H. K.
Stockholm, D.
Stoop, K. W. J.
Storm, T.
Stuckey, D. W.
Stuhmeier, F.
Swoger, J.
Tahir, K. B.
Takagi, M.
Takenaka, S.
Talbot, C.
Talbot, C. B.
Tanimura, A.
Tavernarakis, N.
Taylor, H.
Teraoka, H.
Tojyo, Y.
Torres, M.
Treanor, B.
Truong, K.
Tsien, R. Y.
Ueyama, H.
van Geest, L. K.
Vanveen, J. J. F.
Viellerobe, B.
Vinegoni, C.
Walls, J. R.
Walters, K. B.
Wang, X. F.
Warren, S.
Wasserman, S. C.
Weissleder, R.
Westerberg, C. H.
White, J. G.
Wittbrodt, J.
Wouters, F. S.
Yamashita, S.
Yanik, M. F.
Yoo, S. K.
Yoshizaki, H.
Zhang, W.
Zieglgänsberger, W.
- A. Abbott, “Cell culture: biology’s new dimension,” Nature 424(6951), 870–872 (2003). [CrossRef] [PubMed]
- J. I. Agulleiro and J. J. Fernandez, “Fast tomographic reconstruction on multicore computers,” Bioinformatics 27(4), 582–583 (2011). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- T. P. Barros, W. K. Alderton, H. M. Reynolds, A. G. Roach, and S. Berghmans, “Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery,” Br. J. Pharmacol. 154(7), 1400–1413 (2008). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- M. Oldham, H. S. Sakhalkar, T. Oliver, G. Allan Johnson, and M. Dewhirst, “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography,” J. Biomed. Opt. 13(2), 021113 (2008). [CrossRef] [PubMed]
- T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda, “Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells,” Mol. Biol. Cell 19(10), 4213–4223 (2008). [CrossRef] [PubMed]
- T. Kuner and G. J. Augustine, “A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons,” Neuron 27(3), 447–459 (2000). [CrossRef] [PubMed]
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- R. M. Ballew and J. N. Demas, “An error analysis of the rapid lifetime determination method for the evaluation of single exponential decay,” Anal. Chem. 61(1), 30–33 (1989). [CrossRef]
- T. P. Barros, W. K. Alderton, H. M. Reynolds, A. G. Roach, and S. Berghmans, “Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery,” Br. J. Pharmacol. 154(7), 1400–1413 (2008). [CrossRef] [PubMed]
- D. Stockholm, M. Bartoli, G. Sillon, N. Bourg, J. Davoust, and I. Richard, “Imaging calpain protease activity by multiphoton FRET in living mice,” J. Mol. Biol. 346(1), 215–222 (2005). [CrossRef] [PubMed]
- P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
- D. Beis and D. Y. R. Stainier, “In vivo cell biology: following the zebrafish trend,” Trends Cell Biol. 16(2), 105–112 (2006). [CrossRef] [PubMed]
- T. P. Barros, W. K. Alderton, H. M. Reynolds, A. G. Roach, and S. Berghmans, “Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery,” Br. J. Pharmacol. 154(7), 1400–1413 (2008). [CrossRef] [PubMed]
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- M. Mank, D. F. Reiff, N. Heim, M. W. Friedrich, A. Borst, and O. Griesbeck, “A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change,” Biophys. J. 90(5), 1790–1796 (2006). [CrossRef] [PubMed]
- D. Stockholm, M. Bartoli, G. Sillon, N. Bourg, J. Davoust, and I. Richard, “Imaging calpain protease activity by multiphoton FRET in living mice,” J. Mol. Biol. 346(1), 215–222 (2005). [CrossRef] [PubMed]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- C. G. Burns, D. J. Milan, E. J. Grande, W. Rottbauer, C. A. MacRae, and M. C. Fishman, “High-throughput assay for small molecules that modulate zebrafish embryonic heart rate,” Nat. Chem. Biol. 1(5), 263–264 (2005). [CrossRef] [PubMed]
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
- H. S. Sakhalkar, M. Dewhirst, T. Oliver, Y. Cao, and M. Oldham, “Functional imaging in bulk tissue specimens using optical emission tomography: fluorescence preservation during optical clearing,” Phys. Med. Biol. 52(8), 2035–2054 (2007). [CrossRef] [PubMed]
- D. Castaño Díez, H. Mueller, and A. S. Frangakis, “Implementation and performance evaluation of reconstruction algorithms on graphics processors,” J. Struct. Biol. 157(1), 288–295 (2007). [CrossRef] [PubMed]
- C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010). [CrossRef] [PubMed]
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
- X. F. Wang, A. Periasamy, B. Herman, and D. M. Coleman, “Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications,” Crit. Rev. Anal. Chem. 23(5), 369–395 (1992). [CrossRef]
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- C. Hall, M. V. Flores, T. Storm, K. Crosier, and P. Crosier, “The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish,” BMC Dev. Biol. 7(1), 42 (2007). [CrossRef] [PubMed]
- C. Hall, M. V. Flores, T. Storm, K. Crosier, and P. Crosier, “The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish,” BMC Dev. Biol. 7(1), 42 (2007). [CrossRef] [PubMed]
- K. Dowling, S. C. W. Hyde, J. C. Dainty, P. W. W. French, and J. D. Hares, “2-D fluorescence lifetime imaging using a time-gated image intensifier,” Opt. Commun. 135(1-3), 27–31 (1997). [CrossRef]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- D. Stockholm, M. Bartoli, G. Sillon, N. Bourg, J. Davoust, and I. Richard, “Imaging calpain protease activity by multiphoton FRET in living mice,” J. Mol. Biol. 346(1), 215–222 (2005). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
- R. M. Ballew and J. N. Demas, “An error analysis of the rapid lifetime determination method for the evaluation of single exponential decay,” Anal. Chem. 61(1), 30–33 (1989). [CrossRef]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- M. Oldham, H. S. Sakhalkar, T. Oliver, G. Allan Johnson, and M. Dewhirst, “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography,” J. Biomed. Opt. 13(2), 021113 (2008). [CrossRef] [PubMed]
- H. S. Sakhalkar, M. Dewhirst, T. Oliver, Y. Cao, and M. Oldham, “Functional imaging in bulk tissue specimens using optical emission tomography: fluorescence preservation during optical clearing,” Phys. Med. Biol. 52(8), 2035–2054 (2007). [CrossRef] [PubMed]
- G. Kari, U. Rodeck, and A. P. Dicker, “Zebrafish: an emerging model system for human disease and drug discovery,” Clin. Pharmacol. Ther. 82(1), 70–80 (2007). [CrossRef] [PubMed]
- J. R. Mathias, M. E. Dodd, K. B. Walters, S. K. Yoo, E. A. Ranheim, and A. Huttenlocher, “Characterization of zebrafish larval inflammatory macrophages,” Dev. Comp. Immunol. 33(11), 1212–1217 (2009). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- K. Dowling, M. J. Dayel, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw, “Fluorescence lifetime imaging with picosecond resolution for biomedical applications,” Opt. Lett. 23(10), 810–812 (1998). [CrossRef] [PubMed]
- K. Dowling, S. C. W. Hyde, J. C. Dainty, P. W. W. French, and J. D. Hares, “2-D fluorescence lifetime imaging using a time-gated image intensifier,” Opt. Commun. 135(1-3), 27–31 (1997). [CrossRef]
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- C. Dunsby, “Optically sectioned imaging by oblique plane microscopy,” Opt. Express 16(25), 20306–20316 (2008). [CrossRef] [PubMed]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- A. Esposito, H. C. Gerritsen, T. Oggier, F. Lustenberger, and F. S. Wouters, “Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics,” J. Biomed. Opt. 11(3), 034016 (2006). [CrossRef] [PubMed]
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
- J. I. Agulleiro and J. J. Fernandez, “Fast tomographic reconstruction on multicore computers,” Bioinformatics 27(4), 582–583 (2011). [CrossRef] [PubMed]
- C. G. Burns, D. J. Milan, E. J. Grande, W. Rottbauer, C. A. MacRae, and M. C. Fishman, “High-throughput assay for small molecules that modulate zebrafish embryonic heart rate,” Nat. Chem. Biol. 1(5), 263–264 (2005). [CrossRef] [PubMed]
- C. Hall, M. V. Flores, T. Storm, K. Crosier, and P. Crosier, “The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish,” BMC Dev. Biol. 7(1), 42 (2007). [CrossRef] [PubMed]
- D. Castaño Díez, H. Mueller, and A. S. Frangakis, “Implementation and performance evaluation of reconstruction algorithms on graphics processors,” J. Struct. Biol. 157(1), 288–295 (2007). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- K. Dowling, M. J. Dayel, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw, “Fluorescence lifetime imaging with picosecond resolution for biomedical applications,” Opt. Lett. 23(10), 810–812 (1998). [CrossRef] [PubMed]
- K. Dowling, S. C. W. Hyde, J. C. Dainty, P. W. W. French, and J. D. Hares, “2-D fluorescence lifetime imaging using a time-gated image intensifier,” Opt. Commun. 135(1-3), 27–31 (1997). [CrossRef]
- M. Mank, D. F. Reiff, N. Heim, M. W. Friedrich, A. Borst, and O. Griesbeck, “A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change,” Biophys. J. 90(5), 1790–1796 (2006). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
- A. Esposito, H. C. Gerritsen, T. Oggier, F. Lustenberger, and F. S. Wouters, “Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics,” J. Biomed. Opt. 11(3), 034016 (2006). [CrossRef] [PubMed]
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
- C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010). [CrossRef] [PubMed]
- C. G. Burns, D. J. Milan, E. J. Grande, W. Rottbauer, C. A. MacRae, and M. C. Fishman, “High-throughput assay for small molecules that modulate zebrafish embryonic heart rate,” Nat. Chem. Biol. 1(5), 263–264 (2005). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- M. Mank, D. F. Reiff, N. Heim, M. W. Friedrich, A. Borst, and O. Griesbeck, “A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change,” Biophys. J. 90(5), 1790–1796 (2006). [CrossRef] [PubMed]
- C. Hall, M. V. Flores, T. Storm, K. Crosier, and P. Crosier, “The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish,” BMC Dev. Biol. 7(1), 42 (2007). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- K. Dowling, M. J. Dayel, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw, “Fluorescence lifetime imaging with picosecond resolution for biomedical applications,” Opt. Lett. 23(10), 810–812 (1998). [CrossRef] [PubMed]
- K. Dowling, S. C. W. Hyde, J. C. Dainty, P. W. W. French, and J. D. Hares, “2-D fluorescence lifetime imaging using a time-gated image intensifier,” Opt. Commun. 135(1-3), 27–31 (1997). [CrossRef]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- A. J. Hill, H. Teraoka, W. Heideman, and R. E. Peterson, “Zebrafish as a model vertebrate for investigating chemical toxicity,” Toxicol. Sci. 86(1), 6–19 (2005). [CrossRef] [PubMed]
- M. Mank, D. F. Reiff, N. Heim, M. W. Friedrich, A. Borst, and O. Griesbeck, “A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change,” Biophys. J. 90(5), 1790–1796 (2006). [CrossRef] [PubMed]
- J. R. Walls, J. G. Sled, J. Sharpe, and R. M. Henkelman, “Resolution improvement in emission optical projection tomography,” Phys. Med. Biol. 52(10), 2775–2790 (2007). [CrossRef] [PubMed]
- X. F. Wang, A. Periasamy, B. Herman, and D. M. Coleman, “Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications,” Crit. Rev. Anal. Chem. 23(5), 369–395 (1992). [CrossRef]
- T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda, “Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells,” Mol. Biol. Cell 19(10), 4213–4223 (2008). [CrossRef] [PubMed]
- A. J. Hill, H. Teraoka, W. Heideman, and R. E. Peterson, “Zebrafish as a model vertebrate for investigating chemical toxicity,” Toxicol. Sci. 86(1), 6–19 (2005). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
- J. Huisken and D. Y. R. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32(17), 2608–2610 (2007). [CrossRef] [PubMed]
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
- J. R. Mathias, M. E. Dodd, K. B. Walters, S. K. Yoo, E. A. Ranheim, and A. Huttenlocher, “Characterization of zebrafish larval inflammatory macrophages,” Dev. Comp. Immunol. 33(11), 1212–1217 (2009). [CrossRef] [PubMed]
- K. Dowling, S. C. W. Hyde, J. C. Dainty, P. W. W. French, and J. D. Hares, “2-D fluorescence lifetime imaging using a time-gated image intensifier,” Opt. Commun. 135(1-3), 27–31 (1997). [CrossRef]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- E. A. Jares-Erijman and T. M. Jovin, “FRET imaging,” Nat. Biotechnol. 21(11), 1387–1395 (2003). [CrossRef] [PubMed]
- E. A. Jares-Erijman and T. M. Jovin, “FRET imaging,” Nat. Biotechnol. 21(11), 1387–1395 (2003). [CrossRef] [PubMed]
- G. Kari, U. Rodeck, and A. P. Dicker, “Zebrafish: an emerging model system for human disease and drug discovery,” Clin. Pharmacol. Ther. 82(1), 70–80 (2007). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- P. J. Keller, F. Pampaloni, and E. H. K. Stelzer, “Life sciences require the third dimension,” Curr. Opin. Cell Biol. 18(1), 117–124 (2006). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda, “Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells,” Mol. Biol. Cell 19(10), 4213–4223 (2008). [CrossRef] [PubMed]
- C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- T. Kuner and G. J. Augustine, “A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons,” Neuron 27(3), 447–459 (2000). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
- I. T. Li, E. Pham, and K. Truong, “Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics,” Biotechnol. Lett. 28(24), 1971–1982 (2006). [CrossRef] [PubMed]
- A. Esposito, H. C. Gerritsen, T. Oggier, F. Lustenberger, and F. S. Wouters, “Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics,” J. Biomed. Opt. 11(3), 034016 (2006). [CrossRef] [PubMed]
- C. G. Burns, D. J. Milan, E. J. Grande, W. Rottbauer, C. A. MacRae, and M. C. Fishman, “High-throughput assay for small molecules that modulate zebrafish embryonic heart rate,” Nat. Chem. Biol. 1(5), 263–264 (2005). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
- M. Mank, D. F. Reiff, N. Heim, M. W. Friedrich, A. Borst, and O. Griesbeck, “A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change,” Biophys. J. 90(5), 1790–1796 (2006). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- J. R. Mathias, M. E. Dodd, K. B. Walters, S. K. Yoo, E. A. Ranheim, and A. Huttenlocher, “Characterization of zebrafish larval inflammatory macrophages,” Dev. Comp. Immunol. 33(11), 1212–1217 (2009). [CrossRef] [PubMed]
- T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda, “Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells,” Mol. Biol. Cell 19(10), 4213–4223 (2008). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- C. Parng, W. L. Seng, C. Semino, and P. McGrath, “Zebrafish: a preclinical model for drug screening,” Assay Drug Dev. Technol. 1(1), 41–48 (2002). [CrossRef] [PubMed]
- C. G. Burns, D. J. Milan, E. J. Grande, W. Rottbauer, C. A. MacRae, and M. C. Fishman, “High-throughput assay for small molecules that modulate zebrafish embryonic heart rate,” Nat. Chem. Biol. 1(5), 263–264 (2005). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
- A. Nezu, A. Tanimura, T. Morita, A. Shitara, and Y. Tojyo, “A novel fluorescent method employing the FRET-based biosensor “LIBRA” for the identification of ligands of the inositol 1,4,5-trisphosphate receptors,” Biochim. Biophys. Acta 1760(8), 1274–1280 (2006). [PubMed]
- D. Castaño Díez, H. Mueller, and A. S. Frangakis, “Implementation and performance evaluation of reconstruction algorithms on graphics processors,” J. Struct. Biol. 157(1), 288–295 (2007). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- A. Nezu, A. Tanimura, T. Morita, A. Shitara, and Y. Tojyo, “A novel fluorescent method employing the FRET-based biosensor “LIBRA” for the identification of ligands of the inositol 1,4,5-trisphosphate receptors,” Biochim. Biophys. Acta 1760(8), 1274–1280 (2006). [PubMed]
- T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda, “Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells,” Mol. Biol. Cell 19(10), 4213–4223 (2008). [CrossRef] [PubMed]
- C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2008). [CrossRef] [PubMed]
- R. Weissleder and V. Ntziachristos, “Shedding light onto live molecular targets,” Nat. Med. 9(1), 123–128 (2003). [CrossRef] [PubMed]
- A. Esposito, H. C. Gerritsen, T. Oggier, F. Lustenberger, and F. S. Wouters, “Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics,” J. Biomed. Opt. 11(3), 034016 (2006). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
- M. Oldham, H. S. Sakhalkar, T. Oliver, G. Allan Johnson, and M. Dewhirst, “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography,” J. Biomed. Opt. 13(2), 021113 (2008). [CrossRef] [PubMed]
- H. S. Sakhalkar, M. Dewhirst, T. Oliver, Y. Cao, and M. Oldham, “Functional imaging in bulk tissue specimens using optical emission tomography: fluorescence preservation during optical clearing,” Phys. Med. Biol. 52(8), 2035–2054 (2007). [CrossRef] [PubMed]
- M. Oldham, H. S. Sakhalkar, T. Oliver, G. Allan Johnson, and M. Dewhirst, “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography,” J. Biomed. Opt. 13(2), 021113 (2008). [CrossRef] [PubMed]
- H. S. Sakhalkar, M. Dewhirst, T. Oliver, Y. Cao, and M. Oldham, “Functional imaging in bulk tissue specimens using optical emission tomography: fluorescence preservation during optical clearing,” Phys. Med. Biol. 52(8), 2035–2054 (2007). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
- P. J. Keller, F. Pampaloni, and E. H. K. Stelzer, “Life sciences require the third dimension,” Curr. Opin. Cell Biol. 18(1), 117–124 (2006). [CrossRef] [PubMed]
- C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010). [CrossRef] [PubMed]
- C. Parng, W. L. Seng, C. Semino, and P. McGrath, “Zebrafish: a preclinical model for drug screening,” Assay Drug Dev. Technol. 1(1), 41–48 (2002). [CrossRef] [PubMed]
- X. F. Wang, A. Periasamy, B. Herman, and D. M. Coleman, “Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications,” Crit. Rev. Anal. Chem. 23(5), 369–395 (1992). [CrossRef]
- C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2008). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- A. J. Hill, H. Teraoka, W. Heideman, and R. E. Peterson, “Zebrafish as a model vertebrate for investigating chemical toxicity,” Toxicol. Sci. 86(1), 6–19 (2005). [CrossRef] [PubMed]
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
- I. T. Li, E. Pham, and K. Truong, “Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics,” Biotechnol. Lett. 28(24), 1971–1982 (2006). [CrossRef] [PubMed]
- C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2008). [CrossRef] [PubMed]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
- J. R. Mathias, M. E. Dodd, K. B. Walters, S. K. Yoo, E. A. Ranheim, and A. Huttenlocher, “Characterization of zebrafish larval inflammatory macrophages,” Dev. Comp. Immunol. 33(11), 1212–1217 (2009). [CrossRef] [PubMed]
- C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2008). [CrossRef] [PubMed]
- M. Mank, D. F. Reiff, N. Heim, M. W. Friedrich, A. Borst, and O. Griesbeck, “A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change,” Biophys. J. 90(5), 1790–1796 (2006). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- T. P. Barros, W. K. Alderton, H. M. Reynolds, A. G. Roach, and S. Berghmans, “Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery,” Br. J. Pharmacol. 154(7), 1400–1413 (2008). [CrossRef] [PubMed]
- D. Stockholm, M. Bartoli, G. Sillon, N. Bourg, J. Davoust, and I. Richard, “Imaging calpain protease activity by multiphoton FRET in living mice,” J. Mol. Biol. 346(1), 215–222 (2005). [CrossRef] [PubMed]
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
- T. P. Barros, W. K. Alderton, H. M. Reynolds, A. G. Roach, and S. Berghmans, “Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery,” Br. J. Pharmacol. 154(7), 1400–1413 (2008). [CrossRef] [PubMed]
- G. Kari, U. Rodeck, and A. P. Dicker, “Zebrafish: an emerging model system for human disease and drug discovery,” Clin. Pharmacol. Ther. 82(1), 70–80 (2007). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- C. G. Burns, D. J. Milan, E. J. Grande, W. Rottbauer, C. A. MacRae, and M. C. Fishman, “High-throughput assay for small molecules that modulate zebrafish embryonic heart rate,” Nat. Chem. Biol. 1(5), 263–264 (2005). [CrossRef] [PubMed]
- M. Oldham, H. S. Sakhalkar, T. Oliver, G. Allan Johnson, and M. Dewhirst, “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography,” J. Biomed. Opt. 13(2), 021113 (2008). [CrossRef] [PubMed]
- H. S. Sakhalkar, M. Dewhirst, T. Oliver, Y. Cao, and M. Oldham, “Functional imaging in bulk tissue specimens using optical emission tomography: fluorescence preservation during optical clearing,” Phys. Med. Biol. 52(8), 2035–2054 (2007). [CrossRef] [PubMed]
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- C. Parng, W. L. Seng, C. Semino, and P. McGrath, “Zebrafish: a preclinical model for drug screening,” Assay Drug Dev. Technol. 1(1), 41–48 (2002). [CrossRef] [PubMed]
- C. Parng, W. L. Seng, C. Semino, and P. McGrath, “Zebrafish: a preclinical model for drug screening,” Assay Drug Dev. Technol. 1(1), 41–48 (2002). [CrossRef] [PubMed]
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- J. R. Walls, J. G. Sled, J. Sharpe, and R. M. Henkelman, “Resolution improvement in emission optical projection tomography,” Phys. Med. Biol. 52(10), 2775–2790 (2007). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- A. Nezu, A. Tanimura, T. Morita, A. Shitara, and Y. Tojyo, “A novel fluorescent method employing the FRET-based biosensor “LIBRA” for the identification of ligands of the inositol 1,4,5-trisphosphate receptors,” Biochim. Biophys. Acta 1760(8), 1274–1280 (2006). [PubMed]
- D. Stockholm, M. Bartoli, G. Sillon, N. Bourg, J. Davoust, and I. Richard, “Imaging calpain protease activity by multiphoton FRET in living mice,” J. Mol. Biol. 346(1), 215–222 (2005). [CrossRef] [PubMed]
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
- J. R. Walls, J. G. Sled, J. Sharpe, and R. M. Henkelman, “Resolution improvement in emission optical projection tomography,” Phys. Med. Biol. 52(10), 2775–2790 (2007). [CrossRef] [PubMed]
- P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999). [CrossRef] [PubMed]
- J. Huisken and D. Y. R. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32(17), 2608–2610 (2007). [CrossRef] [PubMed]
- D. Beis and D. Y. R. Stainier, “In vivo cell biology: following the zebrafish trend,” Trends Cell Biol. 16(2), 105–112 (2006). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- P. J. Keller, F. Pampaloni, and E. H. K. Stelzer, “Life sciences require the third dimension,” Curr. Opin. Cell Biol. 18(1), 117–124 (2006). [CrossRef] [PubMed]
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
- D. Stockholm, M. Bartoli, G. Sillon, N. Bourg, J. Davoust, and I. Richard, “Imaging calpain protease activity by multiphoton FRET in living mice,” J. Mol. Biol. 346(1), 215–222 (2005). [CrossRef] [PubMed]
- L. K. van Geest and K. W. J. Stoop, “FLIM on a wide field fluorescence microscope,” Lett. Pept. Sci. 10(5-6), 501–510 (2003). [CrossRef]
- C. Hall, M. V. Flores, T. Storm, K. Crosier, and P. Crosier, “The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish,” BMC Dev. Biol. 7(1), 42 (2007). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- H. Ueyama, M. Takagi, and S. Takenaka, “A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation,” J. Am. Chem. Soc. 124(48), 14286–14287 (2002). [CrossRef] [PubMed]
- H. Ueyama, M. Takagi, and S. Takenaka, “A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation,” J. Am. Chem. Soc. 124(48), 14286–14287 (2002). [CrossRef] [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- A. Nezu, A. Tanimura, T. Morita, A. Shitara, and Y. Tojyo, “A novel fluorescent method employing the FRET-based biosensor “LIBRA” for the identification of ligands of the inositol 1,4,5-trisphosphate receptors,” Biochim. Biophys. Acta 1760(8), 1274–1280 (2006). [PubMed]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- A. J. Hill, H. Teraoka, W. Heideman, and R. E. Peterson, “Zebrafish as a model vertebrate for investigating chemical toxicity,” Toxicol. Sci. 86(1), 6–19 (2005). [CrossRef] [PubMed]
- A. Nezu, A. Tanimura, T. Morita, A. Shitara, and Y. Tojyo, “A novel fluorescent method employing the FRET-based biosensor “LIBRA” for the identification of ligands of the inositol 1,4,5-trisphosphate receptors,” Biochim. Biophys. Acta 1760(8), 1274–1280 (2006). [PubMed]
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- I. T. Li, E. Pham, and K. Truong, “Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics,” Biotechnol. Lett. 28(24), 1971–1982 (2006). [CrossRef] [PubMed]
- R. Y. Tsien, “The green fluorescent protein,” Annu. Rev. Biochem. 67(1), 509–544 (1998). [CrossRef] [PubMed]
- H. Ueyama, M. Takagi, and S. Takenaka, “A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation,” J. Am. Chem. Soc. 124(48), 14286–14287 (2002). [CrossRef] [PubMed]
- L. K. van Geest and K. W. J. Stoop, “FLIM on a wide field fluorescence microscope,” Lett. Pept. Sci. 10(5-6), 501–510 (2003). [CrossRef]
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2008). [CrossRef] [PubMed]
- J. R. Walls, J. G. Sled, J. Sharpe, and R. M. Henkelman, “Resolution improvement in emission optical projection tomography,” Phys. Med. Biol. 52(10), 2775–2790 (2007). [CrossRef] [PubMed]
- J. R. Mathias, M. E. Dodd, K. B. Walters, S. K. Yoo, E. A. Ranheim, and A. Huttenlocher, “Characterization of zebrafish larval inflammatory macrophages,” Dev. Comp. Immunol. 33(11), 1212–1217 (2009). [CrossRef] [PubMed]
- X. F. Wang, A. Periasamy, B. Herman, and D. M. Coleman, “Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications,” Crit. Rev. Anal. Chem. 23(5), 369–395 (1992). [CrossRef]
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010). [CrossRef] [PubMed]
- R. Weissleder and V. Ntziachristos, “Shedding light onto live molecular targets,” Nat. Med. 9(1), 123–128 (2003). [CrossRef] [PubMed]
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
- A. Esposito, H. C. Gerritsen, T. Oggier, F. Lustenberger, and F. S. Wouters, “Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics,” J. Biomed. Opt. 11(3), 034016 (2006). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
- C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010). [CrossRef] [PubMed]
- J. R. Mathias, M. E. Dodd, K. B. Walters, S. K. Yoo, E. A. Ranheim, and A. Huttenlocher, “Characterization of zebrafish larval inflammatory macrophages,” Dev. Comp. Immunol. 33(11), 1212–1217 (2009). [CrossRef] [PubMed]
- T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda, “Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells,” Mol. Biol. Cell 19(10), 4213–4223 (2008). [CrossRef] [PubMed]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
Anal. Chem.
- R. M. Ballew and J. N. Demas, “An error analysis of the rapid lifetime determination method for the evaluation of single exponential decay,” Anal. Chem. 61(1), 30–33 (1989). [CrossRef]
Annu. Rev. Biochem.
- R. Y. Tsien, “The green fluorescent protein,” Annu. Rev. Biochem. 67(1), 509–544 (1998). [CrossRef] [PubMed]
Assay Drug Dev. Technol.
- C. Parng, W. L. Seng, C. Semino, and P. McGrath, “Zebrafish: a preclinical model for drug screening,” Assay Drug Dev. Technol. 1(1), 41–48 (2002). [CrossRef] [PubMed]
Biochim. Biophys. Acta
- A. Nezu, A. Tanimura, T. Morita, A. Shitara, and Y. Tojyo, “A novel fluorescent method employing the FRET-based biosensor “LIBRA” for the identification of ligands of the inositol 1,4,5-trisphosphate receptors,” Biochim. Biophys. Acta 1760(8), 1274–1280 (2006). [PubMed]
Bioinformatics
- J. I. Agulleiro and J. J. Fernandez, “Fast tomographic reconstruction on multicore computers,” Bioinformatics 27(4), 582–583 (2011). [CrossRef] [PubMed]
Biomed. Opt. Express
- V. Y. Soloviev and S. R. Arridge, “Optical Tomography in weakly scattering media in the presence of highly scattering inclusions,” Biomed. Opt. Express 2(3), 440–451 (2011). [CrossRef] [PubMed]
- U. J. Birk, M. Rieckher, N. Konstantinides, A. Darrell, A. Sarasa-Renedo, H. Meyer, N. Tavernarakis, and J. Ripoll, “Correction for specimen movement and rotation errors for in-vivo Optical Projection Tomography,” Biomed. Opt. Express 1(1), 87–96 (2010). [CrossRef] [PubMed]
Biophys. J.
- M. Mank, D. F. Reiff, N. Heim, M. W. Friedrich, A. Borst, and O. Griesbeck, “A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change,” Biophys. J. 90(5), 1790–1796 (2006). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
Biotechnol. Lett.
- I. T. Li, E. Pham, and K. Truong, “Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics,” Biotechnol. Lett. 28(24), 1971–1982 (2006). [CrossRef] [PubMed]
BMC Dev. Biol.
- C. Hall, M. V. Flores, T. Storm, K. Crosier, and P. Crosier, “The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish,” BMC Dev. Biol. 7(1), 42 (2007). [CrossRef] [PubMed]
Br. J. Pharmacol.
- T. P. Barros, W. K. Alderton, H. M. Reynolds, A. G. Roach, and S. Berghmans, “Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery,” Br. J. Pharmacol. 154(7), 1400–1413 (2008). [CrossRef] [PubMed]
ChemPhysChem
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
Clin. Pharmacol. Ther.
- G. Kari, U. Rodeck, and A. P. Dicker, “Zebrafish: an emerging model system for human disease and drug discovery,” Clin. Pharmacol. Ther. 82(1), 70–80 (2007). [CrossRef] [PubMed]
Crit. Rev. Anal. Chem.
- X. F. Wang, A. Periasamy, B. Herman, and D. M. Coleman, “Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications,” Crit. Rev. Anal. Chem. 23(5), 369–395 (1992). [CrossRef]
Curr. Opin. Cell Biol.
- P. J. Keller, F. Pampaloni, and E. H. K. Stelzer, “Life sciences require the third dimension,” Curr. Opin. Cell Biol. 18(1), 117–124 (2006). [CrossRef] [PubMed]
Dev. Comp. Immunol.
- J. R. Mathias, M. E. Dodd, K. B. Walters, S. K. Yoo, E. A. Ranheim, and A. Huttenlocher, “Characterization of zebrafish larval inflammatory macrophages,” Dev. Comp. Immunol. 33(11), 1212–1217 (2009). [CrossRef] [PubMed]
J Biophotonics
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
J. Am. Chem. Soc.
- H. Ueyama, M. Takagi, and S. Takenaka, “A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation,” J. Am. Chem. Soc. 124(48), 14286–14287 (2002). [CrossRef] [PubMed]
J. Biomed. Opt.
- M. Oldham, H. S. Sakhalkar, T. Oliver, G. Allan Johnson, and M. Dewhirst, “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography,” J. Biomed. Opt. 13(2), 021113 (2008). [CrossRef] [PubMed]
- A. Esposito, H. C. Gerritsen, T. Oggier, F. Lustenberger, and F. S. Wouters, “Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics,” J. Biomed. Opt. 11(3), 034016 (2006). [CrossRef] [PubMed]
J. Mol. Biol.
- D. Stockholm, M. Bartoli, G. Sillon, N. Bourg, J. Davoust, and I. Richard, “Imaging calpain protease activity by multiphoton FRET in living mice,” J. Mol. Biol. 346(1), 215–222 (2005). [CrossRef] [PubMed]
J. Phys. D Appl. Phys.
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
J. Struct. Biol.
- D. Castaño Díez, H. Mueller, and A. S. Frangakis, “Implementation and performance evaluation of reconstruction algorithms on graphics processors,” J. Struct. Biol. 157(1), 288–295 (2007). [CrossRef] [PubMed]
Lett. Pept. Sci.
- L. K. van Geest and K. W. J. Stoop, “FLIM on a wide field fluorescence microscope,” Lett. Pept. Sci. 10(5-6), 501–510 (2003). [CrossRef]
Mol. Biol. Cell
- T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda, “Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells,” Mol. Biol. Cell 19(10), 4213–4223 (2008). [CrossRef] [PubMed]
Nat. Biotechnol.
- E. A. Jares-Erijman and T. M. Jovin, “FRET imaging,” Nat. Biotechnol. 21(11), 1387–1395 (2003). [CrossRef] [PubMed]
Nat. Chem. Biol.
- C. G. Burns, D. J. Milan, E. J. Grande, W. Rottbauer, C. A. MacRae, and M. C. Fishman, “High-throughput assay for small molecules that modulate zebrafish embryonic heart rate,” Nat. Chem. Biol. 1(5), 263–264 (2005). [CrossRef] [PubMed]
Nat. Med.
- R. Weissleder and V. Ntziachristos, “Shedding light onto live molecular targets,” Nat. Med. 9(1), 123–128 (2003). [CrossRef] [PubMed]
Nat. Methods
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2008). [CrossRef] [PubMed]
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010). [CrossRef] [PubMed]
Nature
- A. Abbott, “Cell culture: biology’s new dimension,” Nature 424(6951), 870–872 (2003). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
Neuron
- T. Kuner and G. J. Augustine, “A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons,” Neuron 27(3), 447–459 (2000). [CrossRef] [PubMed]
Opt. Commun.
- K. Dowling, S. C. W. Hyde, J. C. Dainty, P. W. W. French, and J. D. Hares, “2-D fluorescence lifetime imaging using a time-gated image intensifier,” Opt. Commun. 135(1-3), 27–31 (1997). [CrossRef]
Opt. Express
- C. Dunsby, “Optically sectioned imaging by oblique plane microscopy,” Opt. Express 16(25), 20306–20316 (2008). [CrossRef] [PubMed]
Opt. Lett.
- J. Huisken and D. Y. R. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32(17), 2608–2610 (2007). [CrossRef] [PubMed]
- K. Dowling, M. J. Dayel, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw, “Fluorescence lifetime imaging with picosecond resolution for biomedical applications,” Opt. Lett. 23(10), 810–812 (1998). [CrossRef] [PubMed]
Phys. Med. Biol.
- J. R. Walls, J. G. Sled, J. Sharpe, and R. M. Henkelman, “Resolution improvement in emission optical projection tomography,” Phys. Med. Biol. 52(10), 2775–2790 (2007). [CrossRef] [PubMed]
- H. S. Sakhalkar, M. Dewhirst, T. Oliver, Y. Cao, and M. Oldham, “Functional imaging in bulk tissue specimens using optical emission tomography: fluorescence preservation during optical clearing,” Phys. Med. Biol. 52(8), 2035–2054 (2007). [CrossRef] [PubMed]
Proc. Natl. Acad. Sci. U.S.A.
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
Scanning
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
Science
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
Toxicol. Sci.
- A. J. Hill, H. Teraoka, W. Heideman, and R. E. Peterson, “Zebrafish as a model vertebrate for investigating chemical toxicity,” Toxicol. Sci. 86(1), 6–19 (2005). [CrossRef] [PubMed]
Trends Cell Biol.
- D. Beis and D. Y. R. Stainier, “In vivo cell biology: following the zebrafish trend,” Trends Cell Biol. 16(2), 105–112 (2006). [CrossRef] [PubMed]
- P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999). [CrossRef] [PubMed]
Zebrafish
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
Other
- A. C. Kak and M. Slaney, “Principles of computerized tomographic imaging,” R. E. O’Malley ed. (SIAM, IEEE Press, New York, 1988).
2011, Kumar, ChemPhysChem
- S. Kumar, D. Alibhai, A. Margineanu, R. Laine, G. Kennedy, J. McGinty, S. Warren, D. Kelly, Y. Alexandrov, I. Munro, C. Talbot, D. W. Stuckey, C. Kimberly, B. Viellerobe, F. Lacombe, E. W.-F. Lam, H. Taylor, M. J. Dallman, G. Stamp, E. J. Murray, F. Stuhmeier, A. Sardini, M. Katan, D. S. Elson, M. A. A. Neil, C. Dunsby, and P. M. W. French, “FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ,” ChemPhysChem 12(3), 609–626 (2011). [CrossRef] [PubMed]
- J. I. Agulleiro and J. J. Fernandez, “Fast tomographic reconstruction on multicore computers,” Bioinformatics 27(4), 582–583 (2011). [CrossRef] [PubMed]
- C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010). [CrossRef] [PubMed]
- A. M. Petzold, V. M. Bedell, N. J. Boczek, J. J. Essner, D. Balciunas, K. J. Clark, and S. C. Ekker, “SCORE imaging: specimen in a corrected optical rotational enclosure,” Zebrafish 7(2), 149–154 (2010). [CrossRef] [PubMed]
- G. T. Kennedy, H. B. Manning, D. S. Elson, M. A. A. Neil, G. W. Stamp, B. Viellerobe, F. Lacombe, C. Dunsby, and P. M. W. French, “A fluorescence lifetime imaging scanning confocal endomicroscope,” J Biophotonics 3(1-2), 103–107 (2010). [CrossRef] [PubMed]
- J. R. Mathias, M. E. Dodd, K. B. Walters, S. K. Yoo, E. A. Ranheim, and A. Huttenlocher, “Characterization of zebrafish larval inflammatory macrophages,” Dev. Comp. Immunol. 33(11), 1212–1217 (2009). [CrossRef] [PubMed]
- J. McGinty, J. Requejo-Isidro, I. Munro, C. B. Talbot, P. A. Kellett, J. D. Hares, C. Dunsby, M A A. Neil, and P. M. W. French, “Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM,” J. Phys. D Appl. Phys. 42(13), 135103 (2009). [CrossRef]
- M. J. Boot, C. H. Westerberg, J. Sanz-Ezquerro, J. Cotterell, R. Schweitzer, M. Torres, and J. Sharpe, “In vitro whole-organ imaging: 4D quantification of growing mouse limb buds,” Nat. Methods 5(7), 609–612 (2008). [CrossRef] [PubMed]
- J. McGinty, K. B. Tahir, R. Laine, C. B. Talbot, C. Dunsby, M. A. A. Neil, L. Quintana, J. Swoger, J. Sharpe, and P. M. W. French, “Fluorescence lifetime optical projection tomography,” J Biophotonics 1(5), 390–394 (2008). [CrossRef] [PubMed]
- T. P. Barros, W. K. Alderton, H. M. Reynolds, A. G. Roach, and S. Berghmans, “Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery,” Br. J. Pharmacol. 154(7), 1400–1413 (2008). [CrossRef] [PubMed]
- C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2008). [CrossRef] [PubMed]
- M. Oldham, H. S. Sakhalkar, T. Oliver, G. Allan Johnson, and M. Dewhirst, “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography,” J. Biomed. Opt. 13(2), 021113 (2008). [CrossRef] [PubMed]
- T. Nishioka, K. Aoki, K. Hikake, H. Yoshizaki, E. Kiyokawa, and M. Matsuda, “Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells,” Mol. Biol. Cell 19(10), 4213–4223 (2008). [CrossRef] [PubMed]
- C. B. Talbot, J. McGinty, D. M. Grant, E. J. McGhee, D. M. Owen, W. Zhang, T. D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, C. Dunsby, M. A. Neil, and P. M. W. French, “High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis,” J Biophotonics 1(6), 514–521 (2008). [CrossRef] [PubMed]
- H. S. Sakhalkar, M. Dewhirst, T. Oliver, Y. Cao, and M. Oldham, “Functional imaging in bulk tissue specimens using optical emission tomography: fluorescence preservation during optical clearing,” Phys. Med. Biol. 52(8), 2035–2054 (2007). [CrossRef] [PubMed]
- H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam, “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia,” Proc. Natl. Acad. Sci. U.S.A. 104(49), 19494–19499 (2007). [CrossRef] [PubMed]
- C. Hall, M. V. Flores, T. Storm, K. Crosier, and P. Crosier, “The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish,” BMC Dev. Biol. 7(1), 42 (2007). [CrossRef] [PubMed]
- G. Kari, U. Rodeck, and A. P. Dicker, “Zebrafish: an emerging model system for human disease and drug discovery,” Clin. Pharmacol. Ther. 82(1), 70–80 (2007). [CrossRef] [PubMed]
- D. Castaño Díez, H. Mueller, and A. S. Frangakis, “Implementation and performance evaluation of reconstruction algorithms on graphics processors,” J. Struct. Biol. 157(1), 288–295 (2007). [CrossRef] [PubMed]
- J. R. Walls, J. G. Sled, J. Sharpe, and R. M. Henkelman, “Resolution improvement in emission optical projection tomography,” Phys. Med. Biol. 52(10), 2775–2790 (2007). [CrossRef] [PubMed]
- D. M. Owen, P. M. P. Lanigan, C. Dunsby, I. Munro, D. Grant, M. A. A. Neil, P. M. W. French, and A. I. Magee, “Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells,” Biophys. J. 90(11), L80–L82 (2006). [CrossRef] [PubMed]
- A. Esposito, H. C. Gerritsen, T. Oggier, F. Lustenberger, and F. S. Wouters, “Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics,” J. Biomed. Opt. 11(3), 034016 (2006). [CrossRef] [PubMed]
- D. Beis and D. Y. R. Stainier, “In vivo cell biology: following the zebrafish trend,” Trends Cell Biol. 16(2), 105–112 (2006). [CrossRef] [PubMed]
- P. J. Keller, F. Pampaloni, and E. H. K. Stelzer, “Life sciences require the third dimension,” Curr. Opin. Cell Biol. 18(1), 117–124 (2006). [CrossRef] [PubMed]
- A. Nezu, A. Tanimura, T. Morita, A. Shitara, and Y. Tojyo, “A novel fluorescent method employing the FRET-based biosensor “LIBRA” for the identification of ligands of the inositol 1,4,5-trisphosphate receptors,” Biochim. Biophys. Acta 1760(8), 1274–1280 (2006). [PubMed]
- I. T. Li, E. Pham, and K. Truong, “Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics,” Biotechnol. Lett. 28(24), 1971–1982 (2006). [CrossRef] [PubMed]
- M. Mank, D. F. Reiff, N. Heim, M. W. Friedrich, A. Borst, and O. Griesbeck, “A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change,” Biophys. J. 90(5), 1790–1796 (2006). [CrossRef] [PubMed]
- C. G. Burns, D. J. Milan, E. J. Grande, W. Rottbauer, C. A. MacRae, and M. C. Fishman, “High-throughput assay for small molecules that modulate zebrafish embryonic heart rate,” Nat. Chem. Biol. 1(5), 263–264 (2005). [CrossRef] [PubMed]
- D. Stockholm, M. Bartoli, G. Sillon, N. Bourg, J. Davoust, and I. Richard, “Imaging calpain protease activity by multiphoton FRET in living mice,” J. Mol. Biol. 346(1), 215–222 (2005). [CrossRef] [PubMed]
- A. J. Hill, H. Teraoka, W. Heideman, and R. E. Peterson, “Zebrafish as a model vertebrate for investigating chemical toxicity,” Toxicol. Sci. 86(1), 6–19 (2005). [CrossRef] [PubMed]
- C. Dunsby, P. M. P. Lanigan, J. McGinty, D. S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F. McCann, B. Treanor, B. Onfelt, D. M. Davis, M. A. A. Neil, and P. M. W. French, “An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy,” J. Phys. D Appl. Phys. 37(23), 3296–3303 (2004). [CrossRef]
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
- E. A. Jares-Erijman and T. M. Jovin, “FRET imaging,” Nat. Biotechnol. 21(11), 1387–1395 (2003). [CrossRef] [PubMed]
- R. Weissleder and V. Ntziachristos, “Shedding light onto live molecular targets,” Nat. Med. 9(1), 123–128 (2003). [CrossRef] [PubMed]
- A. Abbott, “Cell culture: biology’s new dimension,” Nature 424(6951), 870–872 (2003). [CrossRef] [PubMed]
- L. K. van Geest and K. W. J. Stoop, “FLIM on a wide field fluorescence microscope,” Lett. Pept. Sci. 10(5-6), 501–510 (2003). [CrossRef]
- H. Ueyama, M. Takagi, and S. Takenaka, “A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation,” J. Am. Chem. Soc. 124(48), 14286–14287 (2002). [CrossRef] [PubMed]
- J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002). [CrossRef] [PubMed]
- C. Parng, W. L. Seng, C. Semino, and P. McGrath, “Zebrafish: a preclinical model for drug screening,” Assay Drug Dev. Technol. 1(1), 41–48 (2002). [CrossRef] [PubMed]
- N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda, “Spatio-temporal images of growth-factor-induced activation of Ras and Rap1,” Nature 411(6841), 1065–1068 (2001). [CrossRef] [PubMed]
- T. Kuner and G. J. Augustine, “A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons,” Neuron 27(3), 447–459 (2000). [CrossRef] [PubMed]
- P. I. H. Bastiaens and A. Squire, “Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell,” Trends Cell Biol. 9(2), 48–52 (1999). [CrossRef] [PubMed]
- R. Y. Tsien, “The green fluorescent protein,” Annu. Rev. Biochem. 67(1), 509–544 (1998). [CrossRef] [PubMed]
- K. Dowling, S. C. W. Hyde, J. C. Dainty, P. W. W. French, and J. D. Hares, “2-D fluorescence lifetime imaging using a time-gated image intensifier,” Opt. Commun. 135(1-3), 27–31 (1997). [CrossRef]
- E. P. Buurman, R. Sanders, A. Draaijer, H. C. Gerritsen, J. J. F. Vanveen, P. M. Houpt, and Y. K. Levine, “Fluorescence lifetime imaging using a confocal laser scanning microscope,” Scanning 14, 155–159 (1992).
- X. F. Wang, A. Periasamy, B. Herman, and D. M. Coleman, “Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications,” Crit. Rev. Anal. Chem. 23(5), 369–395 (1992). [CrossRef]
- R. M. Ballew and J. N. Demas, “An error analysis of the rapid lifetime determination method for the evaluation of single exponential decay,” Anal. Chem. 61(1), 30–33 (1989). [CrossRef]
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