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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 5 — May. 1, 2011
  • pp: 1359–1365

Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen

Tan Liu, Qing Wei, Jing Wang, Shuliang Jiao, and Hao F. Zhang  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 5, pp. 1359-1365 (2011)
http://dx.doi.org/10.1364/BOE.2.001359


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Abstract

We proposed to measure the metabolic rate of oxygen (MRO2) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the multimodal system to measure flow rate in a phantom made of two capillary tubes of different diameters. We then demonstrated the capability of measuring MRO2 by imaging two parallel vessels selected from the ear of a Swiss Webster mouse. The hemoglobin oxygen saturation (sO2) and the vessel diameter were measured by the LSOR-PAM and the blood flow velocity was measured by the SD-OCT, from which blood flow rate and MRO2 were further calculated. The measured blood flow rates in the two vessels agreed with each other.

© 2011 OSA

OCIS Codes
(110.5120) Imaging systems : Photoacoustic imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Multimodal Imaging

History
Original Manuscript: February 28, 2011
Manuscript Accepted: April 22, 2011
Published: April 27, 2011

Virtual Issues
In vivo Microcirculation Imaging (2011) Biomedical Optics Express

Citation
Tan Liu, Qing Wei, Jing Wang, Shuliang Jiao, and Hao F. Zhang, "Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen," Biomed. Opt. Express 2, 1359-1365 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-5-1359


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