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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 5 — May. 1, 2011
  • pp: 1359–1365

Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen

Tan Liu, Qing Wei, Jing Wang, Shuliang Jiao, and Hao F. Zhang  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 5, pp. 1359-1365 (2011)

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We proposed to measure the metabolic rate of oxygen (MRO2) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the multimodal system to measure flow rate in a phantom made of two capillary tubes of different diameters. We then demonstrated the capability of measuring MRO2 by imaging two parallel vessels selected from the ear of a Swiss Webster mouse. The hemoglobin oxygen saturation (sO2) and the vessel diameter were measured by the LSOR-PAM and the blood flow velocity was measured by the SD-OCT, from which blood flow rate and MRO2 were further calculated. The measured blood flow rates in the two vessels agreed with each other.

© 2011 OSA

OCIS Codes
(110.5120) Imaging systems : Photoacoustic imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Multimodal Imaging

Original Manuscript: February 28, 2011
Manuscript Accepted: April 22, 2011
Published: April 27, 2011

Virtual Issues
In vivo Microcirculation Imaging (2011) Biomedical Optics Express

Tan Liu, Qing Wei, Jing Wang, Shuliang Jiao, and Hao F. Zhang, "Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen," Biomed. Opt. Express 2, 1359-1365 (2011)

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  1. L. Padnick-Silver, J. J. Kang Derwent, E. Giuliano, K. Narfström, and R. A. Linsenmeier, “Retinal oxygenation and oxygen metabolism in Abyssinian cats with a hereditary retinal degeneration,” Invest. Ophthalmol. Vis. Sci. 47(8), 3683–3689 (2006). [CrossRef] [PubMed]
  2. V. A. Alder, E. N. Su, D. Y. Yu, S. J. Cringle, and P. K. Yu, “Diabetic retinopathy: early functional changes,” Clin. Exp. Pharmacol. Physiol. 24(9-10), 785–788 (1997). [CrossRef] [PubMed]
  3. J. Stone, T. Chan-Ling, J. Pe’er, A. Itin, H. Gnessin, and E. Keshet, “Roles of vascular endothelial growth factor and astrocyte degeneration in the genesis of retinopathy of prematurity,” Invest. Ophthalmol. Vis. Sci. 37(2), 290–299 (1996). [PubMed]
  4. R. C. deCharms, “Applications of real-time fMRI,” Nat. Rev. Neurosci. 9(9), 720–729 (2008). [CrossRef] [PubMed]
  5. N. Zhang, X. H. Zhu, Y. Zhang, J. K. Park, and W. Chen, “High-resolution fMRI mapping of ocular dominance layers in cat lateral geniculate nucleus,” Neuroimage 50(4), 1456–1463 (2010). [CrossRef] [PubMed]
  6. S. H. Hardarson, A. Harris, R. A. Karlsson, G. H. Halldorsson, L. Kagemann, E. Rechtman, G. M. Zoega, T. Eysteinsson, J. A. Benediktsson, A. Thorsteinsson, P. K. Jensen, J. Beach, and E. Stefánsson, “Automatic retinal oximetry,” Invest. Ophthalmol. Vis. Sci. 47(11), 5011–5016 (2006). [CrossRef] [PubMed]
  7. A. Harris, R. B. Dinn, L. Kagemann, and E. Rechtman, “A review of methods for human retinal oximetry,” Ophthalmic Surg. Lasers Imaging 34(2), 152–164 (2003). [PubMed]
  8. H. Wehbe, M. Ruggeri, S. Jiao, G. Gregori, C. A. Puliafito, and W. Zhao, “Automatic retinal blood flow calculation using spectral domain optical coherence tomography,” Opt. Express 15(23), 15193–15206 (2007). [CrossRef] [PubMed]
  9. M. Szkulmowski, A. Szkulmowska, T. Bajraszewski, A. Kowalczyk, and M. Wojtkowski, “Flow velocity estimation using joint Spectral and Time domain Optical Coherence Tomography,” Opt. Express 16(9), 6008–6025 (2008). [CrossRef] [PubMed]
  10. Y. K. Tao, K. M. Kennedy, and J. A. Izatt, “Velocity-resolved 3D retinal microvessel imaging using single-pass flow imaging spectral domain optical coherence tomography,” Opt. Express 17(5), 4177–4188 (2009). [CrossRef] [PubMed]
  11. Z. Zhi, W. Cepurna, E. Johnson, T. Shen, J. Morrison, and R. K. Wang, “Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography,” Biomed. Opt. Express 2(3), 579–591 (2011). [CrossRef] [PubMed]
  12. Y. Wang, B. A. Bower, J. A. Izatt, O. Tan, and D. Huang, “In vivo total retinal blood flow measurement by Fourier domain Doppler optical coherence tomography,” J. Biomed. Opt. 12(4), 041215 (2007). [CrossRef] [PubMed]
  13. H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006). [CrossRef] [PubMed]
  14. H. F. Zhang, K. Maslov, and L. V. Wang, “In vivo imaging of subcutaneous structures using functional photoacoustic microscopy,” Nat. Protoc. 2(4), 797–804 (2007). [CrossRef] [PubMed]
  15. H. F. Zhang, K. Maslov, M. Sivaramakrishnan, G. Stoica, and L. V. Wang, “Imaging of hemoglobin oxygen saturation variations in single vessels in vivo using photoacoustic microscopy,” Appl. Phys. Lett. 90(5), 053901 (2007). [CrossRef]
  16. Z. X. Xie, S. L. Jiao, H. F. Zhang, and C. A. Puliafito, “Laser-scanning optical-resolution photoacoustic microscopy,” Opt. Lett. 34(12), 1771–1773 (2009). [CrossRef] [PubMed]
  17. S. L. Jiao, Z. X. Xie, H. F. Zhang, and C. A. Puliafito, “Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography,” Opt. Lett. 34(19), 2961–2963 (2009). [CrossRef] [PubMed]
  18. H. F. Zhang, J. Wang, Q. Wei, T. Liu, S. Jiao, and C. A. Puliafito, “Collecting back-reflected photons in photoacoustic microscopy,” Opt. Express 18(2), 1278–1282 (2010). [CrossRef] [PubMed]
  19. A. Laugier and J. Garai, “Derivation of the Ideal Gas Law,” J. Chem. Educ. 84(11), 1832–1833 (2007). [CrossRef]
  20. H. Fang, K. Maslov, and L. V. Wang, “Photoacoustic Doppler effect from flowing small light-absorbing particles,” Phys. Rev. Lett. 99(18), 184501 (2007). [CrossRef] [PubMed]
  21. J. Yao and L. V. Wang, “Transverse flow imaging based on photoacoustic Doppler bandwidth broadening,” J. Biomed. Opt. 15(2), 021304 (2010). [CrossRef] [PubMed]
  22. R. M. Werkmeister, N. Dragostinoff, M. Pircher, E. Götzinger, C. K. Hitzenberger, R. A. Leitgeb, and L. Schmetterer, “Bidirectional Doppler Fourier-domain optical coherence tomography for measurement of absolute flow velocities in human retinal vessels,” Opt. Lett. 33(24), 2967–2969 (2008). [CrossRef] [PubMed]
  23. M. Sivaramakrishnan, K. Maslov, H. F. Zhang, G. Stoica, and L. V. Wang, “Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels,” Phys. Med. Biol. 52(5), 1349–1361 (2007). [CrossRef] [PubMed]
  24. E. W. Stein, K. Maslov, and L. V. Wang, “Noninvasive, in vivo imaging of blood-oxygenation dynamics within the mouse brain using photoacoustic microscopy,” J. Biomed. Opt. 14(2), 020502 (2009). [CrossRef] [PubMed]
  25. S. Jiao, M. Jiang, J. Hu, A. Fawzi, Q. Zhou, K. K. Shung, C. A. Puliafito, and H. F. Zhang, “Photoacoustic ophthalmoscopy for in vivo retinal imaging,” Opt. Express 18(4), 3967–3972 (2010). [CrossRef] [PubMed]

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