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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 7 — Jul. 1, 2011
  • pp: 1946–1954

SAX microscopy with fluorescent nanodiamond probes for high-resolution fluorescence imaging

Masahito Yamanaka, Yan-Kai Tzeng, Shogo Kawano, Nicholas I. Smith, Satoshi Kawata, Huan-Cheng Chang, and Katsumasa Fujita  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 7, pp. 1946-1954 (2011)
http://dx.doi.org/10.1364/BOE.2.001946


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Abstract

We report the use of fluorescent nanodiamonds (FNDs) as a photostable fluorescent probe for high resolution saturated excitation (SAX) microscopy. We confirmed that FNDs show a nonlinear fluorescence response under saturated excitation conditions generated by intense excitation light. Using FNDs, we quantified the spatial resolution improvement inherent in SAX microscopy, and experimentally demonstrated the scalability of the spatial resolution of SAX microscopy. The photostability of the FNDs allowed us to perform nanoparticle imaging of a multicolor-stained macrophage cell with a spatial resolution beyond the diffraction limit.

© 2011 OSA

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy

ToC Category:
Nanotechnology and Plasmonics

History
Original Manuscript: April 21, 2011
Revised Manuscript: May 27, 2011
Manuscript Accepted: June 10, 2011
Published: June 16, 2011

Citation
Masahito Yamanaka, Yan-Kai Tzeng, Shogo Kawano, Nicholas I. Smith, Satoshi Kawata, Huan-Cheng Chang, and Katsumasa Fujita, "SAX microscopy with fluorescent nanodiamond probes for high-resolution fluorescence imaging," Biomed. Opt. Express 2, 1946-1954 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-7-1946


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