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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 8 — Aug. 1, 2011
  • pp: 2160–2174

Differential diagnosis of breast cancer using quantitative, label-free and molecular vibrational imaging

Yaliang Yang, Fuhai Li, Liang Gao, Zhiyong Wang, Michael J. Thrall, Steven S. Shen, Kelvin K. Wong, and Stephen T. C. Wong  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 8, pp. 2160-2174 (2011)

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We present a label-free, chemically-selective, quantitative imaging strategy to identify breast cancer and differentiate its subtypes using coherent anti-Stokes Raman scattering (CARS) microscopy. Human normal breast tissue, benign proliferative, as well as in situ and invasive carcinomas, were imaged ex vivo. Simply by visualizing cellular and tissue features appearing on CARS images, cancerous lesions can be readily separated from normal tissue and benign proliferative lesion. To further distinguish cancer subtypes, quantitative disease-related features, describing the geometry and distribution of cancer cell nuclei, were extracted and applied to a computerized classification system. The results show that in situ carcinoma was successfully distinguished from invasive carcinoma, while invasive ductal carcinoma (IDC) and invasive lobular carcinoma were also distinguished from each other. Furthermore, 80% of intermediate-grade IDC and 85% of high-grade IDC were correctly distinguished from each other. The proposed quantitative CARS imaging method has the potential to enable rapid diagnosis of breast cancer.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: May 24, 2011
Revised Manuscript: June 24, 2011
Manuscript Accepted: June 29, 2011
Published: July 5, 2011

Yaliang Yang, Fuhai Li, Liang Gao, Zhiyong Wang, Michael J. Thrall, Steven S. Shen, Kelvin K. Wong, and Stephen T. C. Wong, "Differential diagnosis of breast cancer using quantitative, label-free and molecular vibrational imaging," Biomed. Opt. Express 2, 2160-2174 (2011)

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