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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 9 — Sep. 1, 2011
  • pp: 2731–2740

Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection

Rui Zheng, Byung-Wook Park, Dong-Shik Kim, and Brent D. Cameron  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 9, pp. 2731-2740 (2011)

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This paper presents a generally applicable approach for the highly specific detection of blood proteins. Thrombin and thrombin-binding aptamers are chosen for demonstration purposes. The sensor was prepared by immobilizing amine-terminated aptamers onto a gold modified surface using a two-step self-assembled monolayer (SAM) immobilization technique and the physical detection is performed using Surface Plasmon Resonance (SPR). The developed sensor has an optimal detectable range of 5–1000 nM and the results show the sensor has good reversibility, sensitivity and selectivity. Furthermore, the sensor shows the potential of being improved and standardized for direct detection of other blood proteins for clinical applications.

© 2011 OSA

ToC Category:
Biosensors and Molecular Diagnostics

Original Manuscript: July 20, 2011
Revised Manuscript: August 10, 2011
Manuscript Accepted: August 12, 2011
Published: August 31, 2011

Rui Zheng, Byung-Wook Park, Dong-Shik Kim, and Brent D. Cameron, "Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection," Biomed. Opt. Express 2, 2731-2740 (2011)

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