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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 10 — Oct. 1, 2012
  • pp: 2623–2635

In vivo imaging and quantitative analysis of zebrafish embryos by digital holographic microscopy

Jian Gao, Joseph A. Lyon, Daniel P. Szeto, and Jun Chen  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 10, pp. 2623-2635 (2012)

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Digital holographic microscopy (DHM) has been applied extensively to in vitro studies of different living cells. In this paper, we present a novel application of an off-axis DHM system to in vivo study of the development of zebrafish embryos. Even with low magnification microscope objectives, the morphological structures and individual cell types inside developing zebrafish embryos can be clearly observed from reconstructed amplitude images. We further study the dynamic process of blood flow in zebrafish embryos. A calibration routine and post-processing procedures are developed to quantify physiological parameters at different developmental stages. We measure quantitatively the blood flow as well as the heart rate to study the effects of elevated D-glucose (abnormal condition) on circulatory and cardiovascular systems of zebrafish embryos. To enhance our ability to use DHM as a quantitative tool for potential high throughput screening application, the calibration and post-processing algorithms are incorporated into an automated processing software. Our results show that DHM is an excellent non-invasive imaging technique for visualizing the cellular dynamics of organogenesis of zebrafish embryos in vivo.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: June 12, 2012
Revised Manuscript: September 14, 2012
Manuscript Accepted: September 14, 2012
Published: September 20, 2012

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BIOMED 2012 (2012) Biomedical Optics Express

Jian Gao, Joseph A. Lyon, Daniel P. Szeto, and Jun Chen, "In vivo imaging and quantitative analysis of zebrafish embryos by digital holographic microscopy," Biomed. Opt. Express 3, 2623-2635 (2012)

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