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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 11 — Nov. 1, 2012
  • pp: 2752–2760

Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy

Jesse W. Wilson, Simone Degan, Warren S. Warren, and Martin C. Fischer  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 11, pp. 2752-2760 (2012)
http://dx.doi.org/10.1364/BOE.3.002752


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Abstract

Standard histopathology techniques (including paraffin embedding) are incompatible with thick tissue multiphoton imaging, and standard clearing techniques on those specimens destroy some molecular information. We demonstrate multiphoton imaging in specimens prepared according to standard histopathology techniques. This permits unlabeled 3-dimensional histology on archival tissue banks, which is of great value in evaluating prognostic indicators.

© 2012 OSA

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6930) Medical optics and biotechnology : Tissue
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:
Ophthalmology Applications

History
Original Manuscript: August 23, 2012
Revised Manuscript: September 28, 2012
Manuscript Accepted: October 1, 2012
Published: October 3, 2012

Citation
Jesse W. Wilson, Simone Degan, Warren S. Warren, and Martin C. Fischer, "Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy," Biomed. Opt. Express 3, 2752-2760 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-11-2752


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References

  1. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science248(4951), 73–76 (1990). [CrossRef] [PubMed]
  2. W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol.21(11), 1369–1377 (2003). [CrossRef] [PubMed]
  3. W. Min, C. W. Freudiger, S. Lu, and X. S. Xie, “Coherent nonlinear optical imaging: beyond fluorescence microscopy,” Annu. Rev. Phys. Chem.62(1), 507–530 (2011). [CrossRef] [PubMed]
  4. P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, “Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues,” Biophys. J.82(1), 493–508 (2002). [CrossRef] [PubMed]
  5. T. E. Matthews, I. R. Piletic, M. A. Selim, M. J. Simpson, and W. S. Warren, “Pump-probe imaging differentiates melanoma from melanocytic nevi,” Sci. Transl. Med.3(71), 71ra15 (2011). [CrossRef] [PubMed]
  6. A. C. Kwan, K. Duff, G. K. Gouras, and W. W. Webb, “Optical visualization of Alzheimer’s pathology via multiphoton-excited intrinsic fluorescence and second harmonic generation,” Opt. Express17(5), 3679–3689 (2009). [CrossRef] [PubMed]
  7. P. Theer and W. Denk, “On the fundamental imaging-depth limit in two-photon microscopy,” J. Opt. Soc. Am. A23(12), 3139–3149 (2006). [CrossRef] [PubMed]
  8. H. Hama, H. Kurokawa, H. Kawano, R. Ando, T. Shimogori, H. Noda, K. Fukami, A. Sakaue-Sawano, and A. Miyawaki, “Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain,” Nat. Neurosci.14(11), 1481–1488 (2011). [CrossRef] [PubMed]
  9. H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods4(4), 331–336 (2007). [CrossRef] [PubMed]
  10. M. A. Smith, E. L. Barnes, and S. I. Chiosea, “Pathology archive: evaluation of integrity, regulatory compliance, and construction of searchable database from print reports,” Am. J. Clin. Pathol.135(5), 753–759 (2011). [CrossRef] [PubMed]
  11. J. E. Gershenwald, M. I. Colome, J. E. Lee, P. F. Mansfield, C. Tseng, J. J. Lee, C. M. Balch, and M. I. Ross, “Patterns of recurrence following a negative sentinel lymph node biopsy in 243 patients with stage I or II melanoma,” J. Clin. Oncol.16(6), 2253–2260 (1998). [PubMed]
  12. V. V. Tuchin, “Optical clearing of tissues and blood using the immersion method,” J. Phys. D Appl. Phys.38(15), 2497–2518 (2005). [CrossRef]
  13. E. A. Genina, A. N. Bashkatov, and V. V. Tuchin, “Tissue optical immersion clearing,” Expert Rev. Med. Devices7(6), 825–842 (2010). [CrossRef] [PubMed]
  14. S. G. Parra, T. H. Chia, J. P. Zinter, and M. J. Levene, “Multiphoton microscopy of cleared mouse organs,” J. Biomed. Opt.15(3), 036017 (2010). [CrossRef] [PubMed]
  15. A.-S. Chiang, “Aqueous tissue clearing solution,” U.S. patent 6472216 (October 29, 2002).
  16. A. J. Lea, “Solubility of melanins,” Nature170(4330), 709 (1952). [CrossRef] [PubMed]
  17. T. M. Cooper, S. T. Schuschereba, R. D. O’Connor, D. L. Bolton, and D. L. Lund, Melanin: The Effects of Dimethyl Sulfoxide on the Spectral Properties (National Technical Information Service, 1986).
  18. N. Sudheendran, M. Mohamed, M. G. Ghosn, V. V. Tuchin, and K. V. Larin, “Assessment of tissue optical clearing as a function of glucose concentration using optical coherence tomography,” J. Innov. Opt. Health Sci.03(3), 169–176 (2010). [CrossRef] [PubMed]
  19. J. Wang, Y. Liang, S. Zhang, Y. Zhou, H. Ni, and Y. Li, “Evaluation of optical clearing with the combined liquid paraffin and glycerol mixture,” Biomed. Opt. Express2(8), 2329–2338 (2011). [CrossRef] [PubMed]
  20. R. J. Buesa, “Mineral oil: the best xylene substitute for tissue processing yet?” J. Histotechnol.23(2), 143–149 (2000). [CrossRef]
  21. J. Lin, S. H. Kennedy, T. Svarovsky, J. Rogers, J. W. Kemnitz, A. Xu, and K. T. Zondervan, “High-quality genomic DNA extraction from formalin-fixed and paraffin-embedded samples deparaffinized using mineral oil,” Anal. Biochem.395(2), 265–267 (2009). [CrossRef] [PubMed]
  22. S. V. Plotnikov, A. C. Millard, P. J. Campagnola, and W. A. Mohler, “Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres,” Biophys. J.90(2), 693–703 (2006). [CrossRef] [PubMed]
  23. R. M. Zucker, “Whole insect and mammalian embryo imaging with confocal microscopy: morphology and apoptosis,” Cytometry A69A(11), 1143–1152 (2006). [CrossRef] [PubMed]
  24. T. E. Matthews, J. W. Wilson, S. Degan, M. J. Simpson, J. Y. Jin, J. Y. Zhang, and W. S. Warren, “In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature,” Biomed. Opt. Express2(6), 1576–1583 (2011). [CrossRef] [PubMed]
  25. J. W. Wilson, S. Degan, T. Mitropoulos, M. A. Selim, J. Y. Zhang, and W. S. Warren, “In vivo pump-probe microscopy of melanoma and pigmented lesions,” Proc. SPIE8226, 822602, 822602-8 (2012). [CrossRef]
  26. V. Williams and F. Morriss, “Formaldehyde-induced fluorescence as a means for differentiating epinephrine cells from norepinephrine cells in adrenal medulla,” Stain Technol.45(5), 205–213 (1970). [PubMed]
  27. U. Leischner, A. Schierloh, W. Zieglgänsberger, and H.-U. Dodt, “Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples,” PLoS ONE5(4), e10391 (2010). [CrossRef] [PubMed]
  28. S. D. Russell and C. P. Daghlian, “Scanning electron microscopic observations on deembedded biological tissue sections: comparison of different fixatives and embedding materials,” J. Electron Microsc. Tech.2(5), 489–495 (1985). [CrossRef]
  29. A. T. Yeh, B. Choi, J. S. Nelson, and B. J. Tromberg, “Reversible dissociation of collagen in tissues,” J. Invest. Dermatol.121(6), 1332–1335 (2003). [CrossRef] [PubMed]
  30. D. Fu, T. Ye, T. E. Matthews, G. Yurtsever, and W. S. Warren, “Two-color, two-photon, and excited-state absorption microscopy,” J. Biomed. Opt.12(5), 054004 (2007). [CrossRef] [PubMed]
  31. O. Katz, E. Small, Y. Bromberg, and Y. Silberberg, “Focusing and compression of ultrashort pulses through scattering media,” Nat. Photonics5(6), 372–377 (2011). [CrossRef]
  32. K. Isobe, H. Kawano, T. Takeda, A. Suda, A. Kumagai, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Background-free deep imaging by spatial overlap modulation nonlinear optical microscopy,” Biomed. Opt. Express3(7), 1594–1608 (2012). [CrossRef] [PubMed]
  33. D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, “Deep tissue multiphoton microscopy using longer wavelength excitation,” Opt. Express17(16), 13354–13364 (2009). [CrossRef] [PubMed]
  34. C.-K. Sun, C.-C. Chen, S.-W. Chu, T.-H. Tsai, Y.-C. Chen, and B.-L. Lin, “Multiharmonic-generation biopsy of skin,” Opt. Lett.28(24), 2488–2490 (2003). [CrossRef] [PubMed]
  35. P. Mahou, N. Olivier, G. Labroille, L. Duloquin, J.-M. Sintes, N. Peyriéras, R. Legouis, D. Débarre, and E. Beaurepaire, “Combined third-harmonic generation and four-wave mixing microscopy of tissues and embryos,” Biomed. Opt. Express2(10), 2837–2849 (2011). [CrossRef] [PubMed]
  36. P. Samineni, B. Li, J. W. Wilson, W. S. Warren, and M. C. Fischer, “Cross-phase modulation imaging,” Opt. Lett.37(5), 800–802 (2012). [CrossRef] [PubMed]
  37. J. W. Wilson, P. Samineni, W. S. Warren, and M. C. Fischer, “Cross-phase modulation spectral shifting: nonlinear phase contrast in a pump-probe microscope,” Biomed. Opt. Express3(5), 854–862 (2012). [CrossRef] [PubMed]

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Supplementary Material


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