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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 12 — Dec. 1, 2012
  • pp: 3161–3175

Quantitative tomographic imaging of intermolecular FRET in small animals

Vivek Venugopal, Jin Chen, Margarida Barroso, and Xavier Intes  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 12, pp. 3161-3175 (2012)

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Forster resonance energy transfer (FRET) is a nonradiative transfer of energy between two fluorescent molecules (a donor and an acceptor) in nanometer range proximity. FRET imaging methods have been applied to proteomic studies and drug discovery applications based on intermolecular FRET efficiency measurements and stoichiometric measurements of FRET interaction as quantitative parameters of interest. Importantly, FRET provides information about biomolecular interactions at a molecular level, well beyond the diffraction limits of standard microscopy techniques. The application of FRET to small animal imaging will allow biomedical researchers to investigate physiological processes occurring at nanometer range in vivo as well as in situ. In this work a new method for the quantitative reconstruction of FRET measurements in small animals, incorporating a full-field tomographic acquisition system with a Monte Carlo based hierarchical reconstruction scheme, is described and validated in murine models. Our main objective is to estimate the relative concentration of two forms of donor species, i.e., a donor molecule involved in FRETing to an acceptor close by and a nonFRETing donor molecule.

© 2012 OSA

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Image Reconstruction and Inverse Problems

Original Manuscript: August 2, 2012
Revised Manuscript: October 15, 2012
Manuscript Accepted: October 15, 2012
Published: November 8, 2012

Vivek Venugopal, Jin Chen, Margarida Barroso, and Xavier Intes, "Quantitative tomographic imaging of intermolecular FRET in small animals," Biomed. Opt. Express 3, 3161-3175 (2012)

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