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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 2 — Feb. 1, 2012
  • pp: 360–365

Label-free imaging of zebrafish larvae in vivo by photoacoustic microscopy

Shuoqi Ye, Ran Yang, Jingwei Xiong, K. Kirk Shung, Qifa Zhou, Changhui Li, and Qiushi Ren  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 2, pp. 360-365 (2012)

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Zebrafish play an important role in biological and biomedical research. Traditional in vivo imaging methods for studying zebrafish larvae primarily require fluorescence labeling. In this work, relying on tissue intrinsic optical absorption contrast, we acquired high resolution label-free 3D images of zebrafish larvae by using photoacoustic microscopy (PAM) in vivo. The spatial resolution reaches several microns, allowing the study of microstructures in various living organs. We demonstrated that our method has the potential to be a powerful non-invasive imaging method for studying various small animal models, including zebrafish larvae, Caenorhabditis elegans, frogs and drosophila larvae.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Photoacoustic Imaging and Spectroscopy

Original Manuscript: December 23, 2011
Revised Manuscript: January 19, 2012
Manuscript Accepted: January 23, 2012
Published: January 24, 2012

Shuoqi Ye, Ran Yang, Jingwei Xiong, K. Kirk Shung, Qifa Zhou, Changhui Li, and Qiushi Ren, "Label-free imaging of zebrafish larvae in vivo by photoacoustic microscopy," Biomed. Opt. Express 3, 360-365 (2012)

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  1. J. T. Shin and M. C. Fishman, “From zebrafish to human: modular medical models,” Annu. Rev. Genomics Hum. Genet.3(1), 311–340 (2002). [CrossRef] [PubMed]
  2. J. F. Amatruda and E. E. Patton, “Genetic models of cancer in zebrafish,” Int Rev Cell Mol Biol271, 1–34 (2008). [CrossRef] [PubMed]
  3. O. Bandmann and E. A. Burton, “Genetic zebrafish models of neurodegenerative diseases,” Neurobiol. Dis.40(1), 58–65 (2010). [CrossRef] [PubMed]
  4. O. Bandmann, L. Flinn, and H. Mortiboys, “POMD08 Zebrafish models for early onset Parkinson's disease,” J. Neurol. Neurosurg. Psychiatry81(11), e59 (2010). [CrossRef]
  5. C. Zanella, M. Campana, B. Rizzi, C. Melani, G. Sanguinetti, P. Bourgine, K. Mikula, N. Peyrieras, and A. Sarti, “Cells segmentation from 3-D confocal images of early zebrafish embryogenesis,” IEEE Trans. Image Process.19(3), 770–781 (2010). [CrossRef] [PubMed]
  6. M. Kamali, L. J. Day, D. H. Brooks, X. Zhou, and D. M. O’Malley, “Automated identification of neurons in 3D confocal datasets from zebrafish brainstem,” J. Microsc.233(1), 114–131 (2009). [CrossRef] [PubMed]
  7. A. M. Vogel and B. M. Weinstein, “Studying vascular development in the zebrafish,” Trends Cardiovasc. Med.10(8), 352–360 (2000). [CrossRef] [PubMed]
  8. A. Bassi, L. Fieramonti, C. D’Andrea, M. Mione, and G. Valentini, “In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography,” J. Biomed. Opt.16(10), 100502 (2011). [CrossRef] [PubMed]
  9. N. V. Iftimia, D. X. Hammer, R. D. Ferguson, M. Mujat, D. Vu, and A. A. Ferrante, “Dual-beam Fourier domain optical Doppler tomography of zebrafish,” Opt. Express16(18), 13624–13636 (2008). [CrossRef] [PubMed]
  10. L. Kagemann, H. Ishikawa, J. Zou, P. Charukamnoetkanok, G. Wollstein, K. A. Townsend, M. L. Gabriele, N. Bahary, X. Wei, J. G. Fujimoto, and J. S. Schuman, “Repeated, noninvasive, high resolution spectral domain optical coherence tomography imaging of zebrafish embryos,” Mol. Vis.14, 2157–2170 (2008). [PubMed]
  11. K. Divakar Rao, P. Upadhyaya, M. Sharma, and P. K. Gupta, “Noninvasive imaging of ethanol-induced developmental defects in zebrafish embryos using optical coherence tomography,” Birth Defects Res. B Dev. Reprod. Toxicol.n/a (2011), doi:. [CrossRef] [PubMed]
  12. M. Xu and L. V. Wang, “Photoacoustic imaging in biomedicine,” Rev. Sci. Instrum.77(4), 041101 (2006). [CrossRef]
  13. A. Oraevsky and A. Karabutov, “Optoacoustic tomography,” in Biomedical Photonics Handbook, T. Vo-Dinh, ed. (CRC, Boca Raton, FL, 2003), 34–31.
  14. C. Li and L. V. Wang, “Photoacoustic tomography and sensing in biomedicine,” Phys. Med. Biol.54(19), R59–R97 (2009). [CrossRef] [PubMed]
  15. P. Beard, “Biomedical photoacoustic imaging,” Interface Focus1(4), 602–631 (2011). [CrossRef]
  16. D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics3(7), 412–417 (2009). [CrossRef]
  17. S. Gratt, K. Passler, R. Nuster, and G. Paltauf, “Photoacoustic section imaging with an integrating cylindrical detector,” Biomed. Opt. Express2(11), 2973–2981 (2011). [CrossRef] [PubMed]
  18. K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, “Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries,” Opt. Lett.33(9), 929–931 (2008). [CrossRef] [PubMed]
  19. C. Zhang, K. Maslov, and L. V. Wang, “Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo,” Opt. Lett.35(19), 3195–3197 (2010). [CrossRef] [PubMed]
  20. G. Ku, K. Maslov, L. Li, and L. V. Wang, “Photoacoustic microscopy with 2-microm transverse resolution,” J. Biomed. Opt.15(2), 021302 (2010). [CrossRef] [PubMed]
  21. S. Hu and L. V. Wang, “Photoacoustic imaging and characterization of the microvasculature,” J. Biomed. Opt.15(1), 011101 (2010). [CrossRef] [PubMed]
  22. S. Isogai, M. Horiguchi, and B. M. Weinstein, “The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development,” Dev. Biol.230(2), 278–301 (2001). [CrossRef] [PubMed]
  23. “Zebrafish Atlas,” http://zfatlas.psu.edu/ , NIH grant 5R24 RR01744, Jake Gittlen Cancer Research Foundation, and PA Tobacco Settlement Fund.
  24. L. D. Wang, K. Maslov, J. J. Yao, B. Rao, and L. V. Wang, “Fast voice-coil scanning optical-resolution photoacoustic microscopy,” Opt. Lett.36(2), 139–141 (2011). [CrossRef] [PubMed]
  25. S. Jiao, Z. Xie, H. F. Zhang, and C. A. Puliafito, “Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography,” Opt. Lett.34(19), 2961–2963 (2009). [CrossRef] [PubMed]
  26. E. Z. Zhang, B. Povazay, J. Laufer, A. Alex, B. Hofer, B. Pedley, C. Glittenberg, B. Treeby, B. Cox, P. Beard, and W. Drexler, “Multimodal photoacoustic and optical coherence tomography scanner using an all optical detection scheme for 3D morphological skin imaging,” Biomed. Opt. Express2(8), 2202–2215 (2011). [CrossRef] [PubMed]

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