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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 3 — Mar. 1, 2012
  • pp: 612–629

OCT methods for capillary velocimetry

Vivek J. Srinivasan, Harsha Radhakrishnan, Eng H. Lo, Emiri T. Mandeville, James Y. Jiang, Scott Barry, and Alex E. Cable  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 3, pp. 612-629 (2012)
http://dx.doi.org/10.1364/BOE.3.000612


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Abstract

To date, two main categories of OCT techniques have been described for imaging hemodynamics: Doppler OCT and OCT angiography. Doppler OCT can measure axial velocity profiles and flow in arteries and veins, while OCT angiography can determine vascular morphology, tone, and presence or absence of red blood cell (RBC) perfusion. However, neither method can quantify RBC velocity in capillaries, where RBC flow is typically transverse to the probe beam and single-file. Here, we describe new methods that potentially address these limitations. Firstly, we describe a complex-valued OCT signal in terms of a static scattering component, dynamic scattering component, and noise. Secondly, we propose that the time scale of random fluctuations in the dynamic scattering component are related to red blood cell velocity. Analysis was performed along the slow axis of repeated B-scans to parallelize measurements. We correlate our purported velocity measurements against two-photon microscopy measurements of RBC velocity, and investigate changes during hypercapnia. Finally, we image the ischemic stroke penumbra during distal middle cerebral artery occlusion (dMCAO), where OCT velocimetry methods provide additional insight that is not afforded by either Doppler OCT or OCT angiography.

© 2012 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.0180) Medical optics and biotechnology : Microscopy
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5380) Medical optics and biotechnology : Physiology
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: December 1, 2011
Revised Manuscript: February 14, 2012
Manuscript Accepted: February 17, 2012
Published: February 24, 2012

Citation
Vivek J. Srinivasan, Harsha Radhakrishnan, Eng H. Lo, Emiri T. Mandeville, James Y. Jiang, Scott Barry, and Alex E. Cable, "OCT methods for capillary velocimetry," Biomed. Opt. Express 3, 612-629 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-3-612


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