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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 4 — Apr. 1, 2012
  • pp: 777–791

Rapid monitoring of cerebral ischemia dynamics using laser-based optical imaging of blood oxygenation and flow

Hart Levy, Dene Ringuette, and Ofer Levi  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 4, pp. 777-791 (2012)

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Abstract: Imaging blood flow or oxygenation changes using optical techniques is useful for monitoring cortical activity in healthy subjects as well as in diseased states such as stroke or epilepsy. However, in order to gain a better understanding of hemodynamics in conscious, freely moving animals, these techniques must be implemented in a small scale, portable design that is adaptable to a wearable format. We demonstrate a novel system which combines the two techniques of laser speckle contrast imaging and intrinsic optical signal imaging simultaneously, using compact laser sources, to monitor induced cortical ischemia in a full field format with high temporal acquisition rates. We further demonstrate the advantages of using combined measurements of speckle contrast and oxygenation to establish absolute flow velocities, as well as to statistically distinguish between veins and arteries. We accomplish this system using coherence reduction techniques applied to Vertical Cavity Surface Emitting Lasers (VCSELs) operating at 680, 795 and 850 nm. This system uses minimal optical components and can easily be adapted into a portable format for continuous monitoring of cortical hemodynamics.

© 2012 OSA

OCIS Codes
(140.2020) Lasers and laser optics : Diode lasers
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6480) Medical optics and biotechnology : Spectroscopy, speckle

ToC Category:
Neuroscience and Brain Imaging

Original Manuscript: December 22, 2012
Revised Manuscript: February 10, 2012
Manuscript Accepted: March 25, 2012
Published: March 27, 2012

Hart Levy, Dene Ringuette, and Ofer Levi, "Rapid monitoring of cerebral ischemia dynamics using laser-based optical imaging of blood oxygenation and flow," Biomed. Opt. Express 3, 777-791 (2012)

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