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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 6 — Jun. 1, 2012
  • pp: 1162–1172

Reflectance confocal microscopy of optical phantoms

Steven L. Jacques, Bo Wang, and Ravikant Samatham  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 6, pp. 1162-1172 (2012)

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A reflectance confocal scanning laser microscope (rCSLM) operating at 488-nm wavelength imaged three types of optical phantoms: (1) 100-nm-dia. polystyrene microspheres in gel at 2% volume fraction, (2) solid polyurethane phantoms (INO BiomimicTM), and (3) common reflectance standards (SpectralonTM). The noninvasive method measured the exponential decay of reflected signal as the focus (zf) moved deeper into the material. The two experimental values, the attenuation coefficient μ and the pre-exponential factor ρ, were mapped into the material optical scattering properties, the scattering coefficient μs and the anisotropy of scattering g. Results show that μs varies as 58, 8–24, and 130–200 cm-1 for phantom types (1), (2) and (3), respectively. The g varies as 0.112, 0.53–0.67, and 0.003–0.26, respectively.

© 2012 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(290.0290) Scattering : Scattering
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Calibration, Validation and Phantom Studies

Original Manuscript: March 9, 2012
Revised Manuscript: April 27, 2012
Manuscript Accepted: April 27, 2012
Published: May 1, 2012

Virtual Issues
Phantoms for the Performance Evaluation and Validation of Optical Medical Imaging Devices (2012) Biomedical Optics Express
July 27, 2012 Spotlight on Optics

Steven L. Jacques, Bo Wang, and Ravikant Samatham, "Reflectance confocal microscopy of optical phantoms," Biomed. Opt. Express 3, 1162-1172 (2012)

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  1. D. S. Gareau, Ph.D. thesis dissertation, “In vivo confocal microscopy in turbid media” (Oregon Health & Science University, 2006).
  2. S. L. Jacques, D. Levitz, R. Samatham, D. S. Gareau, N. Choudhury, and F. Truffer, “Light scattering in confocal reflectance microscopy,” in Biomedical Applications of Light Scattering, A. Wax and V. Backman, eds. (McGraw-Hill, 2010).
  3. R. Samatham, S. L. Jacques, and P. Campagnola, “Optical properties of mutant versus wild-type mouse skin measured by reflectance-mode confocal scanning laser microscopy (rCSLM),” J. Biomed. Opt.13(4), 041309 (2008). [CrossRef] [PubMed]
  4. D. Levitz, M. T. Hinds, N. Choudhury, N. T. Tran, S. R. Hanson, and S. L. Jacques, “Quantitative characterization of developing collagen gels using optical coherence tomography,” J. Biomed. Opt.15(2), 026019 (2010). [CrossRef] [PubMed]
  5. D. Levitz, M. T. Hinds, A. Ardeshiri, S. R. Hanson, and S. L. Jacques, “Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography,” Biomaterials31(32), 8210–8217 (2010). [CrossRef] [PubMed]
  6. R. Samatham, K. G. Phillips, and S. L. Jacques, “Assessment of optical clearing agents using reflectance-mode confocal scanning laser microscopy,” J. Innovative Opt. Health Sci.03(03), 183–188 (2010). [CrossRef]
  7. S. L. Jacques, “The fractal nature of light scattering,” J. Innovative Opt. Health Sci.04(01), 1–7 (2011). [CrossRef]
  8. R. A. McLaughlin, L. Scolaro, P. Robbins, C. Saunders, S. L. Jacques, and D. D. Sampson, “Parametric imaging of cancer with optical coherence tomography,” J. Biomed. Opt.15(4), 046029 (2010). [CrossRef] [PubMed]
  9. L. Scolaro, R. A. McLaughlin, B. R. Klyen, B. A. Wood, P. D. Robbins, C. M. Saunders, S. L. Jacques, and D. D. Sampson, “Parametric imaging of the local attenuation coefficient in human axillary lymph nodes assessed using optical coherence tomography,” Biomed. Opt. Express3(2), 366–379 (2012). [CrossRef] [PubMed]
  10. INO, Inc., “Biomimic—optical phantoms,” (2012), http://www.ino.ca/en-ca/achievements/description/project-p/optical-phantoms.html .

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