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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 6 — Jun. 1, 2012
  • pp: 1266–1273

Light-induced Ca2+ transients observed in widefield epi-fluorescence microscopy of excitable cells

Alison McDonald, John Harris, Debbi MacMillan, John Dempster, and Gail McConnell  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 6, pp. 1266-1273 (2012)
http://dx.doi.org/10.1364/BOE.3.001266


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Abstract

We have investigated the possibility that variations in the level of intracellular Ca2+ in excitable cells might be induced as an artifact of the incoherent illumination that is being used to monitor transient responses. In order to avoid the fluctuations in power of an arc lamp source, a microscope using a light emitting diode that was calibrated accurately at low power levels, was constructed to provide good control over the dose of light applied to the biological specimen. We report here that higher powers of illumination increased the probability of occurrence of Ca2+ transients even in the sub-mW range normally used to measure such transients in epi-fluorescence work, suggesting that caution should be exercised when designing experiments and interpreting data.

© 2012 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5380) Medical optics and biotechnology : Physiology

ToC Category:
Microscopy

History
Original Manuscript: March 2, 2012
Revised Manuscript: April 25, 2012
Manuscript Accepted: April 25, 2012
Published: May 4, 2012

Citation
Alison McDonald, John Harris, Debbi MacMillan, John Dempster, and Gail McConnell, "Light-induced Ca2+ transients observed in widefield epi-fluorescence microscopy of excitable cells," Biomed. Opt. Express 3, 1266-1273 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-6-1266


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