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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 6 — Jun. 1, 2012
  • pp: 1455–1464

Noninvasive imaging of flowing blood cells using label-free spectrally encoded flow cytometry

Lior Golan, Daniella Yeheskely-Hayon, Limor Minai, Eldad J Dann, and Dvir Yelin  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 6, pp. 1455-1464 (2012)

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Optical microscopy of blood cells in vivo provides a unique opportunity for clinicians and researchers to visualize the morphology and dynamics of circulating cells, but is usually limited by the imaging speed and by the need for exogenous labeling of the cells. Here we present a label-free approach for in vivo flow cytometry of blood using a compact imaging probe that could be adapted for bedside real-time imaging of patients in clinical settings, and demonstrate subcellular resolution imaging of red and white blood cells flowing in the oral mucosa of a human volunteer. By analyzing the large data sets obtained by the system, valuable blood parameters could be extracted and used for direct, reliable assessment of patient physiology.

© 2012 OSA

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1610) Medical optics and biotechnology : Clinical applications
(170.1790) Medical optics and biotechnology : Confocal microscopy

ToC Category:

Original Manuscript: March 9, 2012
Revised Manuscript: May 2, 2012
Manuscript Accepted: May 4, 2012
Published: May 21, 2012

Lior Golan, Daniella Yeheskely-Hayon, Limor Minai, Eldad J Dann, and Dvir Yelin, "Noninvasive imaging of flowing blood cells using label-free spectrally encoded flow cytometry," Biomed. Opt. Express 3, 1455-1464 (2012)

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