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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 8 — Aug. 1, 2012
  • pp: 1757–1773

Optics InfoBase > Biomedical Optics Express > Volume 3 > Issue 8 > Page 1757

Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization

Pinhas Girshovitz and Natan T. Shaked  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 8, pp. 1757-1773 (2012)
http://dx.doi.org/10.1364/BOE.3.001757


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Abstract

We present analysis tools which are formulated using wide-field interferometric phase microscopy measurements, and show their ability to uniquely quantify the life cycle of live cancer cells. These parameters are based directly on the optical path delay profile of the sample and do not necessitate decoupling the refractive index and the thickness in the cell interferometric phase profile, and thus can be calculated using a single-frame acquisition. To demonstrate the use of these parameters, we have constructed a wide-field interferometric phase microscopy setup and closely traced the full lifecycle of HeLa cancer cells. These initial results show the potential of the parameters to distinguish between the different phases of the cell lifecycle, as well others biological phenomena.

© 2012 OSA

OCIS Codes
(090.2880) Holography : Holographic interferometry
(170.1530) Medical optics and biotechnology : Cell analysis
(180.3170) Microscopy : Interference microscopy

ToC Category:
Cell Studies

History
Original Manuscript: April 5, 2012
Revised Manuscript: May 30, 2012
Manuscript Accepted: June 3, 2012
Published: June 29, 2012

Citation
Pinhas Girshovitz and Natan T. Shaked, "Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization," Biomed. Opt. Express 3, 1757-1773 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-8-1757


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