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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 9 — Sep. 1, 2012
  • pp: 2066–2077

Measurement and correction of transverse chromatic offsets for multi-wavelength retinal microscopy in the living eye

Wolf M. Harmening, Pavan Tiruveedhula, Austin Roorda, and Lawrence C. Sincich  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 9, pp. 2066-2077 (2012)
http://dx.doi.org/10.1364/BOE.3.002066


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Abstract

A special challenge arises when pursuing multi-wavelength imaging of retinal tissue in vivo, because the eye’s optics must be used as the main focusing elements, and they introduce significant chromatic dispersion. Here we present an image-based method to measure and correct for the eye’s transverse chromatic aberrations rapidly, non-invasively, and with high precision. We validate the technique against hyperacute psychophysical performance and the standard chromatic human eye model. In vivo correction of chromatic dispersion will enable confocal multi-wavelength images of the living retina to be aligned, and allow targeted chromatic stimulation of the photoreceptor mosaic to be performed accurately with sub-cellular resolution.

© 2012 OSA

OCIS Codes
(170.5810) Medical optics and biotechnology : Scanning microscopy
(330.5510) Vision, color, and visual optics : Psychophysics
(130.2035) Integrated optics : Dispersion compensation devices
(330.7327) Vision, color, and visual optics : Visual optics, ophthalmic instrumentation
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Ophthalmology Applications

History
Original Manuscript: June 12, 2012
Revised Manuscript: August 6, 2012
Manuscript Accepted: August 7, 2012
Published: August 13, 2012

Citation
Wolf M. Harmening, Pavan Tiruveedhula, Austin Roorda, and Lawrence C. Sincich, "Measurement and correction of transverse chromatic offsets for multi-wavelength retinal microscopy in the living eye," Biomed. Opt. Express 3, 2066-2077 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-9-2066


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