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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 9 — Sep. 1, 2012
  • pp: 2190–2199

Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient

Aline Pellizzaro, Gabriel Welker, David Scott, Rance Solomon, James Cooper, Anthony Farone, Mary Farone, Robert S. Mushi, Maria del Pilar Aguinaga, and Daniel Erenso  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 9, pp. 2190-2199 (2012)
http://dx.doi.org/10.1364/BOE.3.002190


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Abstract

Using a laser trap, we have studied the properties of erythrocytes from a sickle cell anemia patient (SCA) after receiving an intravenous blood transfusion, and a normal adult individual carrying normal adult hemoglobin. The hemoglobin type and quantitation assessment was carried out by high performance liquid chromatography (HPLC). We conducted an analysis of the size distributions of the cells. By targeting those erythrocytes in the overlapping regions of size distributions, we have investigated their properties when the cells are trapped and released. The efficacy of the transfusion treatment is also studied by comparing the relative changes in deformation and the relaxation-time of the cells in the two samples.

© 2012 OSA

OCIS Codes
(000.1430) General : Biology and medicine
(000.2190) General : Experimental physics
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Traps, Manipulation, and Tracking

History
Original Manuscript: June 18, 2012
Revised Manuscript: August 15, 2012
Manuscript Accepted: August 16, 2012
Published: August 23, 2012

Citation
Aline Pellizzaro, Gabriel Welker, David Scott, Rance Solomon, James Cooper, Anthony Farone, Mary Farone, Robert S. Mushi, Maria del Pilar Aguinaga, and Daniel Erenso, "Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient," Biomed. Opt. Express 3, 2190-2199 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-9-2190


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