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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 9 — Sep. 1, 2012
  • pp: 2234–2243

Imaging granularity of leukocytes with third harmonic generation microscopy

Cheng-Kun Tsai, Yu-Shing Chen, Pei-Chun Wu, Tsung-Yuan Hsieh, Han-Wen Liu, Chiou-Yueh Yeh, Win-Li Lin, Jean-San Chia, and Tzu-Ming Liu  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 9, pp. 2234-2243 (2012)

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Using third harmonic generation (THG) microscopy, we demonstrate that granularity differences of leukocytes can be revealed without a label. Excited by a 1230 nm femtosecond laser, THG signals were generated at a significantly higher level in neutrophils than other mononuclear cells, whereas signals in agranular lymphocytes were one order of magnitude smaller. Interestingly, the characteristic THG features can also be observed in vivo to track the newly recruited leukocytes following lipopolysaccharide (LPS) challenge. These results suggest that label-free THG imaging may provide timely tracking of leukocyte movement without disturbing the normal cellular or physiological status.

© 2012 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Cell Studies

Original Manuscript: June 27, 2012
Revised Manuscript: August 6, 2012
Manuscript Accepted: August 7, 2012
Published: August 24, 2012

Cheng-Kun Tsai, Yu-Shing Chen, Pei-Chun Wu, Tsung-Yuan Hsieh, Han-Wen Liu, Chiou-Yueh Yeh, Win-Li Lin, Jean-San Chia, and Tzu-Ming Liu, "Imaging granularity of leukocytes with third harmonic generation microscopy," Biomed. Opt. Express 3, 2234-2243 (2012)

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