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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 1 — Jan. 1, 2013
  • pp: 151–159

Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy

Jaepyeong Cha, Jing Zhang, Saumya Gurbani, Gyeong Woo Cheon, Min Li, and Jin U. Kang  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 1, pp. 151-159 (2013)
http://dx.doi.org/10.1364/BOE.4.000151


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Abstract

We report a novel approach to quantitatively assess gene transfection efficacy using dual-modality microendoscopy that can simultaneously monitor both laser scanning reflectance and fluorescence imaging. The system uses a 500-μm-diameter coherent fiber bundle and permits 3.5-μm lateral resolution. Both reflectance and fluorescence images obtained from two silicon avalanche photodetectors are displaying at 1 Hz and processed automatically to calculate gene transfection efficiency (the ratio of fluorescent cells among the total cells). To validate the system performance we examined the expression of cyan fluorescent protein using human cervical cancer cells (HeLa) in four commercially available reagents. The result was compared with that using a high-resolution bench-top microscope.

© 2012 OSA

OCIS Codes
(060.2350) Fiber optics and optical communications : Fiber optics imaging
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy

ToC Category:
Endoscopes, Catheters and Micro-Optics

History
Original Manuscript: November 8, 2012
Revised Manuscript: December 10, 2012
Manuscript Accepted: December 12, 2012
Published: December 18, 2012

Citation
Jaepyeong Cha, Jing Zhang, Saumya Gurbani, Gyeong Woo Cheon, Min Li, and Jin U. Kang, "Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy," Biomed. Opt. Express 4, 151-159 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-1-151


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