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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 11 — Nov. 1, 2013
  • pp: 2347–2361

Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

Annemarie Nadort, Rutger G. Woolthuis, Ton G. van Leeuwen, and Dirk J. Faber  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 11, pp. 2347-2361 (2013)

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We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources.

© 2013 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6480) Medical optics and biotechnology : Spectroscopy, speckle
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Speckle Imaging and Diagnostics

Original Manuscript: July 25, 2013
Revised Manuscript: September 12, 2013
Manuscript Accepted: September 27, 2013
Published: October 7, 2013

Annemarie Nadort, Rutger G. Woolthuis, Ton G. van Leeuwen, and Dirk J. Faber, "Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy," Biomed. Opt. Express 4, 2347-2361 (2013)

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