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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 11 — Nov. 1, 2013
  • pp: 2463–2476

Photoacoustic measurement of refractive index of dye solutions and myoglobin for biosensing applications

Benjamin S. Goldschmidt, Smit Mehta, Jeff Mosley, Chris Walter, Paul J. D. Whiteside, Heather K. Hunt, and John A. Viator  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 11, pp. 2463-2476 (2013)

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Current methods of determining the refractive index of chemicals and materials, such as ellipsometry and reflectometry, are limited by their inability to analyze highly absorbing or highly transparent materials, as well as the required prior knowledge of the sample thickness and estimated refractive index. Here, we present a method of determining the refractive index of solutions using the photoacoustic effect. We show that a photoacoustic refractometer can analyze highly absorbing dye samples to within 0.006 refractive index units of a handheld optical refractometer. Further, we use myoglobin, an early non-invasive biomarker for malignant hyperthermia, as a proof of concept that this technique is applicable for use as a medical diagnostic. Comparison of the speed, cost, simplicity, and accuracy of the techniques shows that this photoacoustic method is well-suited for optically complex systems.

© 2013 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1610) Medical optics and biotechnology : Clinical applications
(240.0240) Optics at surfaces : Optics at surfaces
(240.6490) Optics at surfaces : Spectroscopy, surface
(240.7040) Optics at surfaces : Tunneling
(240.2130) Optics at surfaces : Ellipsometry and polarimetry

ToC Category:
Photoacoustic Imaging and Spectroscopy

Original Manuscript: June 18, 2013
Revised Manuscript: August 20, 2013
Manuscript Accepted: September 9, 2013
Published: October 14, 2013

Benjamin S. Goldschmidt, Smit Mehta, Jeff Mosley, Chris Walter, Paul J. D. Whiteside, Heather K. Hunt, and John A. Viator, "Photoacoustic measurement of refractive index of dye solutions and myoglobin for biosensing applications," Biomed. Opt. Express 4, 2463-2476 (2013)

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