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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 11 — Nov. 1, 2013
  • pp: 2518–2526

Signal and depth enhancement for in vivo flow cytometer measurement of ear skin by optical clearing agents

Yimin Ding, Jing Wang, Zhichao Fan, Dan Wei, Rui Shi, Qingming Luo, Dan Zhu, and Xunbin Wei  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 11, pp. 2518-2526 (2013)
http://dx.doi.org/10.1364/BOE.4.002518


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Abstract

The in vivo flow cytometry (IVFC) has shown a great potential for detecting circulating tumor cells quantitatively in the bloodstream. However, the detection depth suffers from the strong light scattering of tissue. In this study, an innovative ear skin optical clearing agent (ESOCA) is employed to improve the signal quality of the IVFC. Our results show that compared with commonly used glycerol, topical application of ESOCA can enhance the transmittance of rat ear significantly in vivo. The labeled red blood cells can be detected by the IVFC with higher signal quality and greater detection depth. This study is very helpful for potential tumor metastasis studies by the IVFC in deep tissues.

© 2013 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3660) Medical optics and biotechnology : Light propagation in tissues

ToC Category:
Noninvasive Optical Diagnostics

History
Original Manuscript: July 22, 2013
Revised Manuscript: October 4, 2013
Manuscript Accepted: October 10, 2013
Published: October 17, 2013

Citation
Yimin Ding, Jing Wang, Zhichao Fan, Dan Wei, Rui Shi, Qingming Luo, Dan Zhu, and Xunbin Wei, "Signal and depth enhancement for in vivo flow cytometer measurement of ear skin by optical clearing agents," Biomed. Opt. Express 4, 2518-2526 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-11-2518


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References

  1. G. S. Terentyuk, G. N. Maslyakova, L. V. Suleymanova, N. G. Khlebtsov, B. N. Khlebtsov, G. G. Akchurin, I. L. Maksimova, V. V. Tuchin, “Laser-induced tissue hyperthermia mediated by gold nanoparticles: toward cancer phototherapy,” J. Biomed. Opt. 14(2), 021016 (2009). [CrossRef] [PubMed]
  2. W. Lu, Q. Huang, G. Ku, X. Wen, M. Zhou, D. Guzatov, P. Brecht, R. Su, A. Oraevsky, L. V. Wang, C. Li, “Photoacoustic imaging of living mouse brain vasculature using hollow gold nanospheres,” Biomaterials 31(9), 2617–2626 (2010). [CrossRef] [PubMed]
  3. A. Herrmann, M. Kortylewski, M. Kujawski, C. Zhang, K. Reckamp, B. Armstrong, L. Wang, C. Kowolik, J. Deng, R. Figlin, H. Yu, “Targeting Stat3 in the myeloid compartment drastically improves the in vivo antitumor functions of adoptively transferred T cells,” Cancer Res. 70(19), 7455–7464 (2010). [CrossRef] [PubMed]
  4. I. J. Fidler, “The pathogenesis of cancer metastasis: the ‘seed and soil’ hypothesis revisited,” Nat. Rev. Cancer 3(6), 453–458 (2003). [CrossRef] [PubMed]
  5. I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004). [CrossRef] [PubMed]
  6. J. Novak, “Development of in vivo flow cytometry,” Ph.D. dissertation, Dept. Mech. Eng., Harvard Univ. Cambridge, MA, 2004.
  7. Z. C. Fan, J. Yan, G. D. Liu, X. Y. Tan, X. F. Weng, W. Z. Wu, J. Zhou, X. B. Wei, “Real-time monitoring of rare circulating hepatocellular carcinoma cells in an orthotopic model by in vivo flow cytometry Assesses Resection On Metastasis,” Cancer Res. 72(10), 2683–2691 (2012). [CrossRef] [PubMed]
  8. J. Novak, I. Georgakoudi, X. Wei, A. Prossin, C. P. Lin, “In vivo flow cytometer for real-time detection and quantification of circulating cells,” Opt. Lett. 29(1), 77–79 (2004). [CrossRef] [PubMed]
  9. J. Guo, Z. Fan, Z. Gu, X. Wei, “Studying the role of macrophages in circulating prostate cancer cells by in vivo flow cytometry,” J. Innov. Opt. Health Sci. 5(4), 1250027 (2012). [CrossRef]
  10. Y. Li, J. Guo, C. Wang, Z. Fan, G. Liu, C. Wang, Z. Gu, D. Damm, A. Mosig, X. Wei, “Circulation times of prostate cancer and hepatocellular carcinoma cells by in vivo flow cytometry,” Cytometry A 79(10), 848–854 (2011). [CrossRef] [PubMed]
  11. A. N. Bashkatov, E. A. Genina, V. V. Tuchin, “Optical properties of skin, subcutaneous, and muscle tissues: a review,” J. Innov. Opt. Health Sci. 4(1), 9–38 (2011). [CrossRef]
  12. V. V. Tuchin, I. L. Maksimova, D. A. Zimnyakov, I. L. Kon, A. H. Mavlyutov, A. A. Mishin, “Light propagation in tissues with controlled optical properties,” J. Biomed. Opt. 2(4), 401–417 (1997). [CrossRef] [PubMed]
  13. V. V. Tuchin, Optical Clearing of Tissues and Blood, (SPIE Press, Bellingham, 2005), Vol. 154.
  14. V. V. Tuchin, “Optical clearing of tissue and blood using immersion method,” J. Phys. D Appl. Phys. 38(15), 2497–2518 (2005). [CrossRef]
  15. V. V. Tuchin, “Optical immersion as a new tool to control optical properties of tissues and blood,” Laser Phys. 15(8), 1109–1136 (2005).
  16. D. Zhu, K. V. Larin, Q. Luo, V. V. Tuchin, “Recent progress in tissue optical clearing,” Laser Photonics Rev. 7(5), 732–757 (2013). [CrossRef]
  17. X. Wen, Z. Mao, Z. Han, V. V. Tuchin, D. Zhu, “In vivo skin optical clearing by glycerol solutions: mechanism,” J. Biophotonics 3(1-2), 44–52 (2010). [CrossRef] [PubMed]
  18. D. Zhu, J. Wang, Z. Zhi, X. Wen, Q. Luo, “Imaging dermal blood flow through the intact rat skin with an optical clearing method,” J. Biomed. Opt. 15(2), 026008 (2010). [CrossRef] [PubMed]
  19. J. Wang, R. Shi, D. Zhu, “Optical clearing method induced switchable skin window for dermal blood flow imaging,” J. Biomed. Opt. 18(6), 061209 (2013). [CrossRef] [PubMed]
  20. X. Wen, S. L. Jacques, V. V. Tuchin, D. Zhu, “Enhanced optical clearing of skin in vivo and optical coherence tomography in-depth imaging,” J. Biomed. Opt. 17(6), 066022 (2012). [CrossRef] [PubMed]
  21. M. H. Khan, B. Choi, S. Chess, K. M Kelly, J. McCullough, J. S. Nelson, “Optical clearing of in vivo human skin: Implications for light-based diagnostic imaging and therapeutics,” Lasers Surg. Med. 34(2), 83–85 (2004). [CrossRef] [PubMed]
  22. J. Wang, Y. Liang, S. Zhang, Y. Zhou, H. Ni, Y. Li, “Evaluation of optical clearing with the combined liquid paraffin and glycerol mixture,” Biomed. Opt. Express 2(8), 2329–2338 (2011). [CrossRef] [PubMed]
  23. Z. Zhi, Z. Han, Q. Luo, D. Zhu, “Improve optical clearing of skin in vitro with propylene glycol as a penetration enhancer,” J. Innov. Opt. Health Sci. 2(3), 269–278 (2009). [CrossRef]
  24. J. Wang, R. Shi, Y. Zhang, D. Zhu, “Ear skin optical clearing for improving blood flow imaging,” Photon. Lasers Med. 2(1), 37–44 (2013).
  25. S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007). [CrossRef] [PubMed]
  26. Y. Li, Z. Fan, J. Guo, G. Liu, X. Tan, C. Wang et al.., “Circulation times of hepatocellular carcinoma cells by in vivo flow cytometry,” Chin. Opt. Lett. 10, 953–956 (2010).
  27. D. Donoho, “De-noising by soft-thresholding,” IEEE Trans. Inf. Theory 41(3), 613–627 (1995). [CrossRef]
  28. E. A. Genina, A. N. Bashkatov, V. V. Tuchin, “Tissue optical immersion clearing,” Expert Rev. Med. Devices 7(6), 825–842 (2010). [CrossRef] [PubMed]
  29. G. Vargas, A. Readinger, S. S. Dozier, A. J. Welch, “Morphological changes in blood vessels produced by hyperosmotic agents and measured by optical coherence tomography,” Photochem. Photobiol. 77(5), 541–549 (2003). [CrossRef] [PubMed]
  30. H. Cheng, Q. Luo, S. Zeng, S. Chen, W. Luo, H. Gong, “Hyperosmotic chemical agent’s effect on in vivo cerebral blood flow revealed by laser speckle,” Appl. Opt. 43(31), 5772–5777 (2004). [CrossRef] [PubMed]
  31. D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, Q. Luo, “Short-term and long-term effects of optical clearing agents on blood vessels in chick chorioallantoic membrane,” J. Biomed. Opt. 13(2), 021106 (2008). [CrossRef] [PubMed]
  32. C. Liu, Z. Zhi, V. V. Tuchin, Q. Luo, D. Zhu, “Enhancement of skin optical clearing efficacy using photo-irradiation,” Lasers Surg. Med. 42(2), 132–140 (2010). [CrossRef] [PubMed]

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