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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 11 — Nov. 1, 2013
  • pp: 2518–2526

Signal and depth enhancement for in vivo flow cytometer measurement of ear skin by optical clearing agents

Yimin Ding, Jing Wang, Zhichao Fan, Dan Wei, Rui Shi, Qingming Luo, Dan Zhu, and Xunbin Wei  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 11, pp. 2518-2526 (2013)

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The in vivo flow cytometry (IVFC) has shown a great potential for detecting circulating tumor cells quantitatively in the bloodstream. However, the detection depth suffers from the strong light scattering of tissue. In this study, an innovative ear skin optical clearing agent (ESOCA) is employed to improve the signal quality of the IVFC. Our results show that compared with commonly used glycerol, topical application of ESOCA can enhance the transmittance of rat ear significantly in vivo. The labeled red blood cells can be detected by the IVFC with higher signal quality and greater detection depth. This study is very helpful for potential tumor metastasis studies by the IVFC in deep tissues.

© 2013 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3660) Medical optics and biotechnology : Light propagation in tissues

ToC Category:
Noninvasive Optical Diagnostics

Original Manuscript: July 22, 2013
Revised Manuscript: October 4, 2013
Manuscript Accepted: October 10, 2013
Published: October 17, 2013

Yimin Ding, Jing Wang, Zhichao Fan, Dan Wei, Rui Shi, Qingming Luo, Dan Zhu, and Xunbin Wei, "Signal and depth enhancement for in vivo flow cytometer measurement of ear skin by optical clearing agents," Biomed. Opt. Express 4, 2518-2526 (2013)

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