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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 12 — Dec. 1, 2013
  • pp: 2945–2961

Comparison of different metrics for analysis and visualization in spectroscopic optical coherence tomography

Volker Jaedicke, Semih Agcaer, Francisco E. Robles, Marian Steinert, David Jones, Sebastian Goebel, Nils C. Gerhardt, Hubert Welp, and Martin R. Hofmann  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 12, pp. 2945-2961 (2013)
http://dx.doi.org/10.1364/BOE.4.002945


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Abstract

Spectroscopic Optical Coherence Tomography (S-OCT) extracts depth resolved spectra that are inherently available from OCT signals. The back scattered spectra contain useful functional information regarding the sample, since the light is altered by wavelength dependent absorption and scattering caused by chromophores and structures of the sample. Two aspects dominate the performance of S-OCT: (1) the spectral analysis processing method used to obtain the spatially-resolved spectroscopic information and (2) the metrics used to visualize and interpret relevant sample features. In this work, we focus on the second aspect, where we will compare established and novel metrics for S-OCT. These concepts include the adaptation of methods known from multispectral imaging and modern signal processing approaches such as pattern recognition. To compare the performance of the metrics in a quantitative manner, we use phantoms with microsphere scatterers of different sizes that are below the system’s resolution and therefore cannot be differentiated using intensity based OCT images. We show that the analysis of the spectral features can clearly separate areas with different scattering properties in multi-layer phantoms. Finally, we demonstrate the performance of our approach for contrast enhancement in bovine articular cartilage.

© 2013 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(180.0180) Microscopy : Microscopy
(290.5850) Scattering : Scattering, particles
(300.0300) Spectroscopy : Spectroscopy

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: September 24, 2013
Revised Manuscript: November 8, 2013
Manuscript Accepted: November 10, 2013
Published: November 22, 2013

Citation
Volker Jaedicke, Semih Agcaer, Francisco E. Robles, Marian Steinert, David Jones, Sebastian Goebel, Nils C. Gerhardt, Hubert Welp, and Martin R. Hofmann, "Comparison of different metrics for analysis and visualization in spectroscopic optical coherence tomography," Biomed. Opt. Express 4, 2945-2961 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-12-2945


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