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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 2 — Feb. 1, 2013
  • pp: 187–192

Optical detection of nanoparticle-enhanced human papillomavirus genotyping microarrays

Xue Zhe Li, Sookyung Kim, Wonhyung Cho, and Seung-Yop Lee  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 2, pp. 187-192 (2013)
http://dx.doi.org/10.1364/BOE.4.000187


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Abstract

In this study, we propose a new detection method of nanoparticle-enhanced human papillomavirus genotyping microarrays using a DVD optical pick-up with a photodiode. The HPV genotyping DNA chip was labeled using Au/Ag core-shell nanoparticles, prepared on a treatment glass substrate. Then, the bio information of the HPV genotyping target DNA was detected by measuring the difference of the optical signals between the DNA spots and the background parts for cervical cancer diagnosis. Moreover the approximate linear relationship between the concentration of the HPV genotyping target DNA and the optical signal depending on the density of Au/Ag core-shell nanoparticles was obtained by performing a spot finding algorithm. It is shown that the nanoparticle-labeled HPV genotyping target DNA can be measured and quantified by collecting the low-cost photodiode signal on the treatment glass chip, replacing high-cost fluorescence microarray scanners using a photomultiplier tube.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Biosensors and Molecular Diagnostics

History
Original Manuscript: September 10, 2012
Revised Manuscript: December 3, 2012
Manuscript Accepted: December 19, 2012
Published: December 20, 2012

Citation
Xue Zhe Li, Sookyung Kim, Wonhyung Cho, and Seung-Yop Lee, "Optical detection of nanoparticle-enhanced human papillomavirus genotyping microarrays," Biomed. Opt. Express 4, 187-192 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-2-187


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