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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 2 — Feb. 1, 2013
  • pp: 322–330

Targeted vertical cross-sectional imaging with handheld near-infrared dual axes confocal fluorescence endomicroscope

Zhen Qiu, Zhongyao Liu, Xiyu Duan, Supang Khondee, Bishnu Joshi, Michael J. Mandella, Kenn Oldham, Katsuo Kurabayashi, and Thomas D. Wang  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 2, pp. 322-330 (2013)

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We demonstrate vertical cross-sectional (XZ-plane) images of near-infrared (NIR) fluorescence with a handheld dual axes confocal endomicroscope that reveals specific binding of a Cy5.5-labeled peptide to pre-malignant colonic mucosa. This view is perpendicular to the tissue surface, and is similar to that used by pathologists. The scan head is 10 mm in outer diameter (OD), and integrates a one dimensional (1-D) microelectromechanical systems (MEMS) X-axis scanner and a bulky lead zirconate titanate (PZT) based Z-axis actuator. The microscope images in a raster-scanning pattern with a ±6 degrees (mechanical) scan angle at ~3 kHz in the X-axis (fast) and up to 10 Hz (0–400 μm) in the Z-axis (slow). Vertical cross-sectional fluorescence images are collected with a transverse and axial resolution of 4 and 5 μm, respectively, over a field-of-view of 800 μm (width) × 400 μm (depth). NIR vertical cross-sectional fluorescence images of fresh mouse colonic mucosa demonstrate histology-like imaging performance with this miniature instrument.

© 2013 OSA

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.5810) Medical optics and biotechnology : Scanning microscopy

ToC Category:

Original Manuscript: December 10, 2012
Revised Manuscript: January 16, 2013
Manuscript Accepted: January 16, 2013
Published: January 24, 2013

Zhen Qiu, Zhongyao Liu, Xiyu Duan, Supang Khondee, Bishnu Joshi, Michael J. Mandella, Kenn Oldham, Katsuo Kurabayashi, and Thomas D. Wang, "Targeted vertical cross-sectional imaging with handheld near-infrared dual axes confocal fluorescence endomicroscope," Biomed. Opt. Express 4, 322-330 (2013)

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