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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 2 — Feb. 1, 2013
  • pp: 351–363

In vivo imaging of the rodent eye with swept source/Fourier domain OCT

Jonathan J. Liu, Ireneusz Grulkowski, Martin F. Kraus, Benjamin Potsaid, Chen D. Lu, Bernhard Baumann, Jay S. Duker, Joachim Hornegger, and James G. Fujimoto  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 2, pp. 351-363 (2013)

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Swept source/Fourier domain OCT is demonstrated for in vivo imaging of the rodent eye. Using commercial swept laser technology, we developed a prototype OCT imaging system for small animal ocular imaging operating in the 1050 nm wavelength range at an axial scan rate of 100 kHz with ~6 µm axial resolution. The high imaging speed enables volumetric imaging with high axial scan densities, measuring high flow velocities in vessels, and repeated volumetric imaging over time. The 1050 nm wavelength light provides increased penetration into tissue compared to standard commercial OCT systems at 850 nm. The long imaging range enables multiple operating modes for imaging the retina, posterior eye, as well as anterior eye and full eye length. A registration algorithm using orthogonally scanned OCT volumetric data sets which can correct motion on a per A-scan basis is applied to compensate motion and merge motion corrected volumetric data for enhanced OCT image quality. Ultrahigh speed swept source OCT is a promising technique for imaging the rodent eye, proving comprehensive information on the cornea, anterior segment, lens, vitreous, posterior segment, retina and choroid.

© 2013 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Ophthalmology Applications

Original Manuscript: December 11, 2012
Revised Manuscript: January 14, 2013
Manuscript Accepted: January 18, 2013
Published: January 29, 2013

Jonathan J. Liu, Ireneusz Grulkowski, Martin F. Kraus, Benjamin Potsaid, Chen D. Lu, Bernhard Baumann, Jay S. Duker, Joachim Hornegger, and James G. Fujimoto, "In vivo imaging of the rodent eye with swept source/Fourier domain OCT," Biomed. Opt. Express 4, 351-363 (2013)

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