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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 4 — Apr. 1, 2013
  • pp: 481–489

Sensitive and specific discrimination of pathogenic and nonpathogenic Escherichia coli using Raman spectroscopy—a comparison of two multivariate analysis techniques

Khozima Hamasha, Qassem I. Mohaidat, Russell A. Putnam, Ryan C. Woodman, Sunil Palchaudhuri, and Steven J. Rehse  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 4, pp. 481-489 (2013)

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The determination of bacterial identity at the strain level is still a complex and time-consuming endeavor. In this study, visible wavelength spontaneous Raman spectroscopy has been used for the discrimination of four closely related Escherichia coli strains: pathogenic enterohemorrhagic E. coli O157:H7 and non-pathogenic E. coli C, E. coli Hfr K-12, and E. coli HF4714. Raman spectra from 600 to 2000 cm−1 were analyzed with two multivariate chemometric techniques, principal component-discriminant function analysis and partial least squares-discriminant analysis, to determine optimal parameters for the discrimination of pathogenic E. coli from the non-pathogenic strains. Spectral preprocessing techniques such as smoothing with windows of various sizes and differentiation were investigated. The sensitivity and specificity of both techniques was in excess of 95%, determined by external testing of the chemometric models. This study suggests that spontaneous Raman spectroscopy with visible wavelength excitation is potentially useful for the rapid identification and classification of clinically-relevant bacteria at the strain level.

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OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1580) Medical optics and biotechnology : Chemometrics
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(300.6450) Spectroscopy : Spectroscopy, Raman

ToC Category:
Spectroscopic Diagnostics

Original Manuscript: December 21, 2012
Revised Manuscript: February 15, 2013
Manuscript Accepted: February 19, 2013
Published: March 1, 2013

Khozima Hamasha, Qassem I. Mohaidat, Russell A. Putnam, Ryan C. Woodman, Sunil Palchaudhuri, and Steven J. Rehse, "Sensitive and specific discrimination of pathogenic and nonpathogenic Escherichia coli using Raman spectroscopy—a comparison of two multivariate analysis techniques," Biomed. Opt. Express 4, 481-489 (2013)

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