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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 4 — Apr. 1, 2013
  • pp: 531–537

Quantification of PpIX concentration in basal cell carcinoma and squamous cell carcinoma models using spatial frequency domain imaging

Ulas Sunar, Daniel J. Rohrbach, Janet Morgan, Natalie Zeitouni, and Barbara W. Henderson  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 4, pp. 531-537 (2013)

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5-aminolaevulinic acid photodynamic therapy (ALA-PDT) is an attractive treatment option for nonmelanoma skin tumors, especially for multiple lesions and large areas. The efficacy of ALA-PDT is highly dependent on the photosensitizer (PS) concentration present in the tumor. Thus it is desirable to quantify PS concentration and distribution, preferably noninvasively to determine potential outcome. Here we quantified protoporphyrin IX (PpIX) distribution induced by topical and intra-tumoral (it) administration of the prodrug ALA in basal and squamous cell carcinoma murine models by using spatial frequency domain imaging (SFDI). The in vivo measurements were validated by analysis of the ex vivo extraction of PpIX. The study demonstrates the feasibility of non-invasive quantification of PpIX distributions in skin tumors.

© 2013 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5180) Medical optics and biotechnology : Photodynamic therapy

ToC Category:
Optics in Cancer Research

Original Manuscript: January 15, 2013
Revised Manuscript: March 5, 2013
Manuscript Accepted: March 5, 2013
Published: March 6, 2013

Ulas Sunar, Daniel J. Rohrbach, Janet Morgan, Natalie Zeitouni, and Barbara W. Henderson, "Quantification of PpIX concentration in basal cell carcinoma and squamous cell carcinoma models using spatial frequency domain imaging," Biomed. Opt. Express 4, 531-537 (2013)

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