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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 4 — Apr. 1, 2013
  • pp: 538–547

Stokes vector based polarization resolved second harmonic microscopy of starch granules

Nirmal Mazumder, Jianjun Qiu, Matthew R. Foreman, Carlos Macías Romero, Peter Török, and Fu-Jen Kao  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 4, pp. 538-547 (2013)

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We report on the measurement and analysis of the polarization state of second harmonic signals generated by starch granules, using a four-channel photon counting based Stokes-polarimeter. Various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), and anisotropy are extracted from the 2D second harmonic Stokes images of starch granules. The concentric shell structure of a starch granule forms a natural photonic crystal structure. By integration over all the solid angle, it will allow very similar SHG quantum efficiency regardless of the angle or the states of incident polarization. Given type I phase matching and the concentric shell structure of a starch granule, one can easily infer the polarization states of the input beam from the resulting SH micrograph.

© 2013 OSA

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.5410) Instrumentation, measurement, and metrology : Polarimetry
(320.0320) Ultrafast optics : Ultrafast optics
(160.1435) Materials : Biomaterials
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Spectroscopic Diagnostics

Original Manuscript: January 7, 2013
Revised Manuscript: February 23, 2013
Manuscript Accepted: February 23, 2013
Published: March 11, 2013

Nirmal Mazumder, Jianjun Qiu, Matthew R. Foreman, Carlos Macías Romero, Peter Török, and Fu-Jen Kao, "Stokes vector based polarization resolved second harmonic microscopy of starch granules," Biomed. Opt. Express 4, 538-547 (2013)

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