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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 6 — Jun. 1, 2013
  • pp: 842–851

Characterizing optical properties of nano contrast agents by using cross-referencing OCT imaging

Jiefeng Xi, Yongping Chen, and Xingde Li  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 6, pp. 842-851 (2013)

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We report a cross-referencing method to quickly and accurately characterize the optical properties of nanoparticles including the extinction, scattering, absorption and backscattering cross sections by using an OCT system alone. Among other applications, such a method is particularly useful for developing nanoparticle-based OCT imaging contrast agents. The method involves comparing two depth-dependent OCT intensity signals collected from two samples (with one having and the other not having the nanoparticles), to extract the extinction and backscattering coefficient, from which the absorption coefficient can be further deduced (with the help of the established scattering theories for predicting the ratio of the backscattering to total scattering cross section). The method has been experimentally validated using test nanoparticles and was then applied to characterizing gold nanocages. With the aid of this method, we were able to successfully synthesize scattering dominant gold nanocages for the first time and demonstrated the highest contrast enhancement ever achieved by the gold nanocages (and by any nanoparticles of a similar size and concentration) in an in vivo mouse tumor model. This method also enables quantitative analysis of contrast enhancement and provides a general guideline on choosing the optimal concentration and optical properties for the nanoparticle-based OCT contrast agents.

© 2013 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(290.0290) Scattering : Scattering
(290.5820) Scattering : Scattering measurements

ToC Category:
Optical Coherence Tomography

Original Manuscript: March 26, 2013
Revised Manuscript: May 6, 2013
Manuscript Accepted: May 7, 2013
Published: May 9, 2013

Jiefeng Xi, Yongping Chen, and Xingde Li, "Characterizing optical properties of nano contrast agents by using cross-referencing OCT imaging," Biomed. Opt. Express 4, 842-851 (2013)

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