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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 7 — Jul. 1, 2013
  • pp: 1074–1082

Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications

Eben Olson, Richard Torres, and Michael J. Levene  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 7, pp. 1074-1082 (2013)
http://dx.doi.org/10.1364/BOE.4.001074


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Abstract

We describe an optical system which reduces the cost and complexity of fluorescence correlation spectroscopy (FCS), intended to increase the suitability of the technique for clinical use. Integration of the focusing optics and sample chamber into a plastic component produces a design which is simple to align and operate. We validate the system by measurements on fluorescent dye, and compare the results to a commercial instrument. In addition, we demonstrate its application to measurements of concentration and multimerization of the clinically relevant protein von Willebrand factor (vWF) in human plasma.

© 2013 OSA

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(220.0220) Optical design and fabrication : Optical design and fabrication

ToC Category:
Clinical Instrumentation

History
Original Manuscript: March 27, 2013
Revised Manuscript: May 30, 2013
Manuscript Accepted: May 30, 2013
Published: June 11, 2013

Citation
Eben Olson, Richard Torres, and Michael J. Levene, "Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications," Biomed. Opt. Express 4, 1074-1082 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-7-1074


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References

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