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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 7 — Jul. 1, 2013
  • pp: 1091–1103

Multiple contrast metrics from the measurements of a digital confocal microscope

Alexandre S. Goy, Michaël Unser, and Demetri Psaltis  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 7, pp. 1091-1103 (2013)
http://dx.doi.org/10.1364/BOE.4.001091


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Abstract

We describe various methods to process the data collected with a digital confocal microscope (DCM) in order to get more information than what we could get from a conventional confocal system. Different metrics can be extracted from the data collected with the DCM in order to produce images that reveal different features of the sample. The integrated phase of the scattered field allows for the three-dimensional reconstruction of the refractive index distribution. In a similar way, the integration of the field intensity yields the absorption coefficient distribution. The deflection of the digitally reconstructed focus reveals the sample-induced aberrations and the RMS width of the focus gives an indication on the local scattering coefficient. Finally, in addition to the conventional confocal metric, which consists in integrating the intensity within the pinhole, the DCM allows for the measurement of the phase within the pinhole. This metrics is close to the whole-field integrated phase and thus gives a qualitative image of the refractive index distribution.

© 2013 OSA

OCIS Codes
(110.6960) Imaging systems : Tomography
(180.1790) Microscopy : Confocal microscopy
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: March 28, 2013
Revised Manuscript: May 30, 2013
Manuscript Accepted: June 3, 2013
Published: June 12, 2013

Citation
Alexandre S. Goy, Michaël Unser, and Demetri Psaltis, "Multiple contrast metrics from the measurements of a digital confocal microscope," Biomed. Opt. Express 4, 1091-1103 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-7-1091


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